纳米银/脱细胞真皮基质的制备及抗菌效果评价

Preparation of nano-silver /xenogenic acellular dermal matrix and its antibacterial evaluation

目的探讨含纳米银的脱细胞真皮基质的制备方法,以解决脱细胞真皮基质无抗菌活性的缺点;通过各种方法评价新型复合敷料的抗菌效果。方法通过倍比稀释法和基内接种法测定纳米银溶液的最低抑菌浓度和最低杀菌浓度;将脱细胞真皮基质浸渍在不同浓度的纳米银溶液中一定时间,制备出含不同浓度纳米银的复合敷料;通过Kirby-Baucer方法比较不同浓度浸渍的脱细胞真皮基质的抑菌效果。结果纳米银溶液对金黄色葡萄球菌的最低抑菌浓度是8mg/L,最低杀菌浓度是64mg/L;纳米银溶液对铜绿假单胞菌的最低抑菌浓度8mg/L,最低杀菌浓度是32mg/L。浸渍吸附法制备出含有不同浓度纳米银的脱细胞真皮基质。纳米银/脱细胞真皮基质对金黄色葡萄球菌和铜绿假单胞菌的抑菌效果优于纳米银医用抗菌敷料。结论纳米银/脱细胞真皮基质拥有良好的抗菌和杀菌效果,可望成为具有抗菌活性的生物敷料。

Objective A convenient preparation method to combine nano-silver with acellular dermal matrix （ADM） is introduced and it can enhance the antibacterial properties of ADM. The antibacterial effects of this composite biological dressing are assesed comparing with the tradition simple dressing. Methods The minimal inhibitory concentration （MIC） and minimum bactericidal concentration （MBC） of nano-silver solution were detected by double dilution method and medium inoculation method respectively. The ADM was soaked into nano-silver solution of different concentrations for a certain time to get nano-silver/ADM. In Kirby-Bancer method, the antibacterial properties were assessed by comparing nano-silver/ADM that soaked in different concentrations of nano-silver. Results The MIC and MBC of nano-silver solution to Staphylococcus anreus were 8 mg/L and 64 mg/L respectively, while the MIC and MBC to Pseudomonas aeruginosa were 8 mg/L and 32 mg/L respectively. Nano-silver/ADM compound dressing was got. Nano-silver/ADM was superior to Aikexin in the antibacterial effects on either Staphylococcus aureus or Pseudomonas aeruginosa. Conclusions Nano-silver/ADM has both the biologicalactivity of ADM and the excellent antibacterial effect of nano-silver. Nano-silver/ADM is a clinically useful antibacterial and biological composite dressing.