一个麻疯树AP2/ERF家族基因的克隆和植物表达载体的构建

Cloning of An AP2/EFR Family Gene from Jatropha curcas L. and Construction of Its Plant Expression Vector

[目的]克隆1个麻疯树AP2/EFR家族的基因,并构建其植物表达载体。[方法]利用麻疯树EST数据库中的AP2/ERF相关信息设计引物,通过RT-PCR克隆麻疯树AP2/EFR家族的基因,用生物信息学方法对其序列进行分析,并构建其植物表达载体。[结果]试验克隆到1个AP2/EFR家族基因JcERF;从cDNA序列、氨基酸序列、保守域序列、功能组成、理化性质、进化树等方面进行分析,结果显示JcERF属于AP2/EFR家族中的ERF亚家族;将JcERF连接至pCAMBIA1301载体上,构建了以35S为启动子的植物表达载体。[结论]该研究为麻疯树中JcERF的功能研究及提高麻疯树的抗逆性奠定了基础。

[ Objective] The paper was to clone an AP2/EFR family gene from Jatropha curcas L. and to construct its plant expression vector. [ Method] The AP2/ERF related information in J. curcas EST database was used to design primers and the AP2/EFR family genes were cloned by RT - PCR. The sequence of gene was analyzed by bioinformatics methods and its plant expression vector was constructed. [ Result] An AP2/EFR family gene JcERF was cloned; the cDNA sequence, amino acid sequence, conserved domain sequence, function composition, physicochemical properties, phylogenetic trees were analyzed, and the results showed that the JcERF gene belonged to the ERF subfamily of AP2/EFR family; the JcERF was connected to pCAMBIA1301 vector and a plant expression vector was constructed with a promoter of 35S. [ Conclusionl The study laid a foundation for the function research of .IcERF in J. curcas and the imnrovement of its resistance to stress.