摘要
[目的]探讨厚朴种子生活力的快速测定方法。[方法]采用四唑染色法(TTC法),设置不同的TTC浓度、染色温度和时间,研究厚朴种子生活力测定的适宜条件。[结果]TTC法测定厚朴种子生活力的适宜条件为:采用浓度0.2%的TTC溶液,在55℃的水浴中染色50 min。[结论]该法可作为厚朴种子生活力测定的有效方法。
[ Objective] To study on rapid determination method of seed viability of Magnolia officinalis Rehd. et Wils. [ Method] Using TTC method and setting different TTC concentration, dyeing temperature and dyeing time, the suitable conditions of Magnolia officinalis seed viabil- ity determination were studied. [ Result] The optimal determination conditions for the viability of Magnolia officinalis seed is that staining for 50min in 55 ℃ water bath with 0.5% Trc solution. [ Conclusion] This is an effective method to be used to determine Magnolia officinalis seed viability.
出处
《安徽农业科学》
CAS
2013年第13期5706-5706,5730,共2页
Journal of Anhui Agricultural Sciences
基金
湖北省教育厅科研重点项目(项目编号D20121607)
关键词
厚朴种子
TTC法
种子生活力
Magnolia officinalis Rehd.et Wils.
TTC method
Seed viability
