NF-κB在狼疮肾炎中的作用

Expression of transcription of NF-κB in renal tissue of lupus nephritis

目的 了解狼疮肾炎 (LN)肾组织中NF κB表达并探讨其在LN中的作用。方法 以NF κB亚基 p6 5单抗为抗体 ,采用微波免疫组织化学法 (APAAP法 )染色 ,检测LN肾组织NF κB表达 ,并进一步分析其与LN活动指数、肾脏病理和功能损害的关系。结果 狼疮肾炎组织中NF κB表达较正常肾组织显著增高 ,尤以WHOⅣ型为甚。NF κB在肾小球和肾小管均有表达 ,但以肾小管表达更明显。LN肾组织NF κB阳性细胞数与肾组织活动指数、肾脏病理改变和肾功能损害显著相关。结论 NF κB可能参与了LN的发病机制 ,检测其在肾组织中的表达可作为反映狼疮肾组织活动病变和进行性肾损害的参考指标。

Objective To investigate the role of NF κB in the pathogenesis of lupus nephritis (LN).Method A microwave based immunohistochemistry (APAAP) was used to detect the expression of NF κB p65 in renal tissue of LN and normal control.At the same time,the correlation between expression of NF κB and renal injuries in LN was examined.Results The expression of NF κB in LN was higher than that of normal control,especially in WHO IV LN.The number of positive cell expressed NF κB in renal tissue of LN showed a strong correlation with kidney activity of LN histological and functional changes.Conclusion NF κB may play an important role in the pathogenesis of LN,and the number of positive cell expressed NF κB may be considered as a predictor for LN,activity and progressive renal injury.