新建1例人肺鳞癌细胞系

Establishment of human lung carcinoma cell line HB-99

目的建立人肺癌肿瘤细胞系，为肿瘤的研究工作提供重要的材料。方法采用手术标本的组织块培养法，建立了１例新的肺癌细胞系ＨＢ－９９，并做初步的生物学特征鉴定。结果该细胞系呈单层贴壁生长，用相差显微镜和电镜分析，其具有细胞的多形性，细胞倍增时间为２４ｈ，克隆形成率４０％，染色体易位复杂，众数６３～６５。异体移植实验显示在裸鼠体内生长的肿块具有与原始手术标本相似的组织形态。结论根据细胞系的特征显示该细胞系是一新建的肺癌细胞系。

Objective To establish a human lung carcinoma cell line and it will be useful in the research of lung cancer. Methods A new human lung carcinoma cell line, designed HB-99, was established by primary culture and resected specimen was from a male patient with lung squamous cancer. Results This cell line grew in monolayers, exhibited cellular morphology as observed by phase contrast and electron microscopy. It had a doubling time of 24 hours, and a cloning efficiency of 40%. Chromosomal analysis showed complicated rearrangements with a modal number of 63 - 65. Conclusion The characterization suggests that the cell line is really a newly established one.