HPLC法测定10%噻唑磷颗粒剂含量

Determination on the Content of 10% Fosthiazate Granule by HPLC

建立了高效液相色谱法测定10%噻唑磷颗粒剂有效成分含量的方法。采用岛津CLC-ODS（150 mm×6.0 mm,5μm）色谱柱;柱温为30℃;流动相为V（甲醇）∶V（水）=65∶35;流速为1.0 mL/min;检测波长为220 nm;噻唑磷保留时间为7.0 min。结果表明标准样品溶液进样量在0.053 5～0.855 8 mg/mL时线性关系良好（r=0.999 9、n=5）,平均回收率为99.12-99.96%,RSD为0.23%。该方法专属性强,重现性好,准确度高,可用于10%噻唑磷颗粒剂有效成分的含量测定。

In order to determine the effective components of 10% fosthiazate granule,the Shimadzu CLC-ODS colunmn（150 mm×6.0 mm,5 μm） was selected,column temperature was 30 ℃,methanol water（65∶35） was selected as the mobile phase,the flow rate was 1.0 mL/min and the detection wavelength was 320 nm,the retention time of fosthiazate was 7.0 min.The results showed that linear relationship was good（r = 0.999 9,n = 5） in the range of 0.053 5~0.855 8 mg/mL,the average recovery rate was 99.12%~99.96% and the RSD was 0.23%.This method was specific,reproducible and accurate to determine the effective components of 10% fosthiazate granule.