摘要
采用高效液相色谱法,准确、快速简便地测定盐酸乙胺丁醇胶囊的含量。色谱条件为:HPX-87C钙离子交换色谱柱、示差折光检测器、流动相为超纯水、柱温70℃,按照外标法测定盐酸乙胺丁醇胶囊的含量。结果表明,试验验证该方法专属性良好,盐酸乙胺丁醇浓度在0.5～5 mg/mL范围内的线性良好,相关系数为0.999 3,回收率为99.6%,RSD=0.42%,且具有良好的稳定性,检验结果与药典方法相比无明显差异,并具有操作简便、快速的特点。该法适用于盐酸乙胺丁醇胶囊含量的测定。
Using HPLC method,the content of ethambutol hydrochloride capsule was determined rapidly and simply.The chromatographic conditions were that selecting HPX-87C column and refractive index detector,the mobile phase was ultra pure water,the column temperature was 70 ℃.The content of ethambutol hydrochloride capsules was detemined with the external standard method.The results showed that the specificity of the method was good,ethambutol hydrochloride concentration linear in the range of 0.5~5 mg/mL was good,the correlation coefficient was 0.999 3,the recovery rate was 99.6%,RSD was 0.42%,and it had good stability,the results between this method and the Pharmacopoeia method had no obvious difference,and had the features of simplicity and speediness,could be suitable for the determination of the content of ethambutol hydrochloride capsule.
出处
《煤炭与化工》
CAS
2013年第2期142-143,150,共3页
Coal and Chemical Industry
