种公牛口蹄疫免疫抗体跟踪监测报告

Report on immune antibody monitoring of FMD in Bull

为探索目前使用的口蹄疫O型-亚I双价灭活苗对种公牛的免疫效果,本试验对某场的种公牛连续17个月的口蹄疫O-亚I双价苗免疫抗体进行跟踪监测,每2个月采集1次血清,用液相阻断ELISA法检测O型和亚洲I型口蹄疫免疫抗体水平,科学评估同一厂家生产的同一毒株疫苗对不同品种牛的保护期以及免疫抗体水平与牛的品种、年龄关联性,进行"差别化"免疫,确保每头种公牛都能获得有效的免疫保护。结果表明,抗体水平与牛的年龄无明显关联性;O型口蹄疫的免疫效果优于亚洲I型;不同品种牛由于遗传上的差异,对疫苗免疫的反应不同,其中,疫苗免疫对HS种公牛的保护效果最差;总体来说,O型和亚洲I型疫苗免疫后,对种公牛的有效保护期可维持在10个月以上。

To explore the immune effect of the currently used foot-and-mouth dis- ease virus（FMDV） type andsian I bivalent inactivated vaccine of bulls, We choosed a breeding oxen farm to carry out the tracking detection of the immune anti- bodies after vaccination. We gathered the serums of the immunized oxen every two months to detect the immune antibodies by liquid phase blocking ELISA in a period of 17 months, Meanwhile, according to the results of the detection of antibodies, we can scientifically evaluate the protection period aim to different kinds of bulls which injected by the same vaccine strain from a company, and investivate whether the immune antibodies have some relationship with the breed or age of the immunized bulls. The results showed that the antibodies levels with no relationship with the age of the bull; The immune antibodies level of type is better than typesian I; The immune response to the vaccine is different due to the genetic diverisity of different kinds of bulls, whereas the H$ bulls had the lowest protection level after vaccination. Overall, the protection period can be maintained in 10 months after vac cination of type and sian I bivalent inactivated vaccine of bulls.