烟草中一种糖苷的分离鉴定及其热解产物

Isolation and Identification of a New Glycoside in Tobacco and Analysis of its Pyrolytic Products

通过95%乙醇提取,常压和高压制备色谱分离,从红花大金元烟叶中提取分离得到化合物Ⅰ,利用质谱和核磁共振技术鉴定了化合物Ⅰ的结构,并在氦气中进行了热裂解-GC/MS分析。结果表明:①化合物Ⅰ为(E)-N-[(4-羟基-3-甲氧基)苯乙基]-3-[(3-甲氧基-4-β-D-吡喃葡萄糖)苯基]丙烯酰胺;②化合物Ⅰ的热解产物主要是4-乙烯基愈创木酚、愈创木酚、香兰素、对甲基苯酚、2,3-二氢苯并呋喃、4-烯丙基苯酚和3-乙基苯酚。

A new glycoside,（E）-N-[（4-hydroxy-3-methoxy）-phenethyl]-3-[（3-methoxy-4-O-β-D-glucopyranosyloxy） phenyl]acrylamide（Ⅰ）,was isolated from leaves of flue-cured tobacco cv.＂Hongda＂through extraction with 95% ethanol,preparation at normal and high pressure and separation with chromatography.The molecular structure of compound（Ⅰ）was identified with HRESI-MS and NMR.Compound（Ⅰ）was pyrolyzed in He atmosphere and its pyrolytic products were analyzed by GC/MS.The results showed that the separated product was compound（Ⅰ）,and its major pyrolytic products were 4-vinylguaiacol,guaiacol,vanillin,p-cresol,2,3-dihydrobenzofuran,4-allylphenol,and m-ethylphenol.