溶剂萃取-中心切割多维色谱-质谱法测定烟草主要中性香味成分

Determination of Main Neutral Aroma Components in Tobacco by Solvent Extraction with Heart-cut Multidimensional Gas Chromatography-Mass Spectrometry

采用溶剂萃取-中心切割多维气相色谱-质谱技术（MDGC/MS）建立了一种烟草主要中性香味成分的分析方法。0.5 g烟样经5 mL乙醚萃取和2 mL 5%H2SO4酸洗,静置过夜后萃取液用多维气相色谱-质谱分析;以甲基丁子香酚为内标,对茄酮、β-大马酮、香叶基丙酮、β-紫罗兰酮、巨豆三烯酮和二氢猕猴桃内酯等中性香味成分进行了定性定量测定,并与同时蒸馏萃取（SDE）-气质联用（GC/MS）法进行比较。结果表明：各待测组分检出限在0.02~0.18μg/mL之间,回收率为80.0%~123.4%,日内相对标准偏差≤7.5%。该方法快速、简便、准确,适用于烟草中性香味成分的批量测试。

A method for determining the main neutral aroma components in tobacco was developed by combining solvent extraction with heart-cut multidimensional gas chromatography-mass spectrometry（MDGC-MS）.Tobacco sample（0.5 g）was extracted by ethyl ether（5 mL）,the extract was washed with 5% H2SO4（2 mL）subsequently,then analyzed by MDGC-MS after standing overnight.The neutral aromatic components,including solanone,β-damascenone,geranylacetone,β-ionone,megastigmatrienone and dihydroactinidiolide were determined qualitatively and quantitatively by using methyl eugenol as the internal standard.Further,this method was compared with simultaneous distillation extraction（SDE）-gas chromatography-mass spectrometry（GC/MS）.The results showed that： The detection limit of the analytes ranged from 0.02 to 0.18 μg/mL,the recoveries were between 80.0% to 123.4% with the intra-day relative standard deviation（RSD）≤7.5%.The developed method is simple,fast,accurate and suitable for the analysis of neutral aroma compounds in batches of tobacco samples.