疏肝健脾方对腹泻型肠易激综合征大鼠结肠黏膜5-羟色胺及其受体的影响

Effects of Shuganjianpi prescription on the 5-HT and its receptors of colon mucosa in D-IBS rats

目的观察疏肝健脾方对腹泻型肠易激综合征(D-IBS)模型大鼠结肠黏膜5-羟色胺(5-HT),5-羟色胺3受体(5-HT3R)及5-羟色胺4受体(5-HT4R)表达的影响。方法将60只SD雄性大鼠随机分为正常组、模型组、得舒特组和疏肝健脾高、中、低剂量组,除正常组外其余各组使用乙酸灌肠加束缚应激的方法制备D-IBS大鼠模型,造模成功后各组分别予生理盐水、得舒特、疏肝健脾方灌胃2周。取大鼠结肠黏膜,采用酶联免疫法检测5-HT,免疫组化法检测5-HT3R及5-HT4R的表达。结果疏肝健脾方可以明显降低D-IBS大鼠粪便含水量(P<0.01);疏肝健脾方可以明显增加D-IBS大鼠的最小容量阈值(P<0.01);疏肝健脾方可以降低D-IBS大鼠结肠黏膜5-HT和5-HT3R的表达(P<0.01),升高5-HT4R的表达(P<0.01)。结论疏肝健脾方对D-IBS模型大鼠的治疗作用可能是通过调节结肠5-HT及其受体,减弱神经元兴奋性、提高内脏痛阈、消除肠道敏感而达到的。

Objective To investigate the effects of Shuganjianpi prescription（SGJP） on the expression of 5-hydroxytryptamine（5-HT）,5-HT3receptor（5-HT3R） and 5-HT4receptor（5-HT4R） of colon mucosa in the irritable bowel syndrome-diarrhea predominant（D-IBS） rats.Methods Sixty male Sprague-Dawley（SD） rats were randomly divided into six groups： a normal group,a model group,a Pinaverium Bromide（PB） group and a high-,medium-and low-dose of SGJP group（Group H,M and L）.All groups except the normal group received enema of acetic acid and restraint stress for preparation of D-IBS model,which were intragastric administrated with saline,PB,and SGJP for two weeks respectively after modeling successfully.The colon mucosa of rats was taken for detecting the expression of 5-HT,5-HT3R and 5-HT4R by enzymelinked immunosorbent assay and immunohistochemical assay.Results The SGJP significantly reduced the fecal water content of D-IBS rats（P 0.01）;the SGJP significantly increased the minimum capacity threshold of D-IBS rats（P 0.01）;the SGJP significantly reduced the expression of 5-HT and 5-HT3R of colon mucosa in D-IBS rats（P 0.01）,while significantly increased expression of 5-HT4R（P 0.01）.Conclusion The therapeutic action of SGJP on the D-IBS rats might be realized through regulating 5-HT and its receptors,reducing neuronal excitability,increasing visceral pain threshold and eliminating intestinal sensitivity.