石榴花色苷近红外光谱定量分析模型

Modeling for Quantitative Analysis of Anthocyanins in Pomegranate by Near-infrared Spectroscopy (NIRS)

采用InfraXact型多功能近红外分析仪,利用正交试验设计研究不同预处理和校正方法对建模效果的影响,建立石榴中花色苷定标模型,利用目标函数法对模型进行评定,并对评定的最优模型适用性进行验证。结果表明:在最优组合处理下,模型的验证决定系数为0.958,相对标准差为6.599%,相对分析误差为4.970。说明近红外光谱定量分析石榴中花色苷的含量是可行的,模型的稳定性和预测能力较好。

This paper describes the development of a model for near-infrared spectral quantitative analysis of anthocyanins in pomegranate.Pomegranate was tested with an InfraXact NIR analyzer.The effects of different pretreatment and calibration methods on modeling were studied by orthogonal array design.A calibration model was developed and evaluated by objective function method and the optimized model was validated with respect to applicability.Under optimal conditions,the coefficient of determination was 0.958,RSD 6.599%,and RPD 4.970.These results showed that NIRS could provide a feasible method for quantitative analysis of anthocyanins in pomegranate and the model established in this study had good stability and prediction ability.