摘要
为了提高内生细菌解淀粉芽孢杆菌的抗菌代谢物质的产生,结合均匀设计(UD)与支持向量回归(SVR)发展了一种新的配方优化方法UD-SVR,用来优化选择培养基成分和发酵罐条件。最优配方考虑9因素通过3轮混合水平实验得出最佳优化条件为:淀粉0.5%、葡萄糖2%、酵母提取物0.4%、CaCl21.3%、KH2PO40.1%、30℃、36h、接种量1%、pH7.0。发酵液稀释10倍抑制活性达到57.39%,比基准实验高出20.53%,UD-SVR明显优于参比模型,可被广泛应用于实验设计和分析的处方优化过程中。
In order to increase the production of antimicrobial metabolites against Alternaria alternate by an antagonistic endophytic strain L(Bacillus amyloliquefaciens),a novel experimental design and analysis approach,uniform design combined with support vector regression(UD-SVR),was proposed to optimize culture medium components and fermenter conditions.Three rounds of mixed level experiments of nine variables showed that the optimal culture medium consisted of starch 0.5%,glucose 2%,yeast extract 0.4%,CaCl 2 1.3% and KH 2 PO 4 0.1% at pH 7.0,and the optimal fermentation conditions were 1% of inoculum amount,30 ℃ and 36 h.The antimicrobial activity of a 10-fold dilution of the fermentation broth obtained under the optimized conditions was 57.39%,a 20.53% increase in comparison with the value from benchmark experiments.In conclusion,UD-SVR is more efficient than the reference models and has more promising potential for wide applications in experimental design and optimization.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第13期144-151,共8页
Food Science
基金
国家自然科学基金项目(31071738
31000827)
湖南省教育厅重点项目(10A051)
湖南省自然科学基金项目(09JJ-3032)
湖南农业大学稳定人才科学基金项目(07WDl8)
关键词
优化
均匀设计与支持向量机
解淀粉芽孢杆菌
抑菌活性
optimization
uniform design combined with support vector regression
Bacillus amyloliquefaciens
inhibitory activity