基于均匀设计与支持向量回归的拮抗辣椒链格孢菌解淀粉芽孢杆菌发酵条件优化(英文)

Optimization of Fermentation Conditions for an Antagonistic Endophytic Bacillus amyloliquefaciens Strain L for Inhibition of Alternaria alternata in Chilli by Uniform Design and Support Vector Regression

为了提高内生细菌解淀粉芽孢杆菌的抗菌代谢物质的产生,结合均匀设计(UD)与支持向量回归(SVR)发展了一种新的配方优化方法UD-SVR,用来优化选择培养基成分和发酵罐条件。最优配方考虑9因素通过3轮混合水平实验得出最佳优化条件为:淀粉0.5%、葡萄糖2%、酵母提取物0.4%、CaCl21.3%、KH2PO40.1%、30℃、36h、接种量1%、pH7.0。发酵液稀释10倍抑制活性达到57.39%,比基准实验高出20.53%,UD-SVR明显优于参比模型,可被广泛应用于实验设计和分析的处方优化过程中。

In order to increase the production of antimicrobial metabolites against Alternaria alternate by an antagonistic endophytic strain L（Bacillus amyloliquefaciens）,a novel experimental design and analysis approach,uniform design combined with support vector regression（UD-SVR）,was proposed to optimize culture medium components and fermenter conditions.Three rounds of mixed level experiments of nine variables showed that the optimal culture medium consisted of starch 0.5%,glucose 2%,yeast extract 0.4%,CaCl 2 1.3% and KH 2 PO 4 0.1% at pH 7.0,and the optimal fermentation conditions were 1% of inoculum amount,30 ℃ and 36 h.The antimicrobial activity of a 10-fold dilution of the fermentation broth obtained under the optimized conditions was 57.39%,a 20.53% increase in comparison with the value from benchmark experiments.In conclusion,UD-SVR is more efficient than the reference models and has more promising potential for wide applications in experimental design and optimization.