摘要
以玉米麸质为原料,通过响应面法对中性蛋白酶酶解玉米蛋白的条件进行优化并建立回归模型,得到最佳水解工艺条件为:酶解时间3.4h、酶与底物比1.25%、酶解pH 6.95、料液比1:23(m/V),此工艺条件下的水解度预测值为16.51%,水解度实测值为(16.38±0.11)%。在最优条件下制备得到的玉米蛋白酶解液对乙醇脱氢酶的激活率为(21.23±0.17)%,氨基酸测定结果表明玉米蛋白酶解液中的丙氨酸和亮氨酸的含量较高。
Protein hydrolysis of defatted corn gluten by neutrase was optimized using response surface methodology in this study.Results showed that the best hydrolysis conditions were found to be 3.4 h,1.25%,6.95 and 1：23（m/V） for hydrolysis time,enzyme/substrate ratio,pH and material/liquid ratio,respectively.Under these conditions,the predicted and actual values of degree of hydrolysis（DH） were 16.51% and（16.38 ± 0.11）%,respectively.The corn protein hydrolysate prepared under the optimal conditions activated alcohol dehydrogenase by（21.23 ± 0.17）%.Amino acid analysis showed higher contents of alanine and leucine in the hydrolysate.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第13期170-174,共5页
Food Science
基金
教育部东北林业大学大学生创新训练项目(11102250018)
关键词
玉米蛋白
中性蛋白酶
水解
响应面
乙醇脱氢酶
corn proteins
neutrase
hydrolysis
response surface methodology
alcohol dehydrogenase
