莲房原花青素对极低频电磁场致海马神经元损伤的预防作用

Preventive Effect of Procyanidins from Lotus Seedpod on Hippocampal Neurons Injury Induced by Extremely Low Frequency Electromagnetic Field

研究极低频电磁场(ELF-EMF)对原代培养的大鼠海马神经元的损伤作用及莲房原花青素(LSPC)的预防作用。采用原代培养建立海马神经元极低频电磁场损伤模型及LSPC预处理模型细胞,检测神经细胞生存率、细胞形态、海马神经元细胞内活性氧(ROS)含量。结果表明:2.5、5、10μg/mL LSPC预处理组的细胞生存率分别为81.23%、90.18%和93.45%,明显高于模型组(66.88%,P<0.05);ELF-EMF引起神经元细胞内ROS含量上升(荧光强度(228.19±16.28)),经2.5、5、10μg/mL LSPC预处理后细胞内ROS荧光强度显著下降(P<0.05)。由此提示LSPC对ELF-EMF导致的大鼠海马神经元损伤有明显的预防作用。

To investigate the preventive effect of procyanidins from lotus seedpod（LSPC） on hippocampal neuron injury induced by extremely low frequency electromagnetic fields（ELF-EMF）,primary cultured hippocampal neurons from rats were pretreated with LSPC before exposure to ELF-EMF.Survival rates,the morphology of neurons and the level of reactive oxygen species（ROS） were measured.The survival rates of hippocampal neurons pretreated with LSPC（2.5,5 μg/mL and 10 μg/mL） were 81.23%,90.18% and 93.45%,respectively,which were significantly higher than those from the model group（66.88%,P 0.05）.Electromagnetic radiation increased the ROS level in neurons（the fluorescence intensity was（228.19 ± 16.28）,while pretreatment with 2.5,5,10 μg/mL of LSPC reduced ROS level obviously（P 0.05）.All these studies indicated pretreatment with LSPC could significantly block hippocampal neuron injury induced by extremely low frequency electromagnetic field.