摘要
研究极低频电磁场(ELF-EMF)对原代培养的大鼠海马神经元的损伤作用及莲房原花青素(LSPC)的预防作用。采用原代培养建立海马神经元极低频电磁场损伤模型及LSPC预处理模型细胞,检测神经细胞生存率、细胞形态、海马神经元细胞内活性氧(ROS)含量。结果表明:2.5、5、10μg/mL LSPC预处理组的细胞生存率分别为81.23%、90.18%和93.45%,明显高于模型组(66.88%,P<0.05);ELF-EMF引起神经元细胞内ROS含量上升(荧光强度(228.19±16.28)),经2.5、5、10μg/mL LSPC预处理后细胞内ROS荧光强度显著下降(P<0.05)。由此提示LSPC对ELF-EMF导致的大鼠海马神经元损伤有明显的预防作用。
To investigate the preventive effect of procyanidins from lotus seedpod(LSPC) on hippocampal neuron injury induced by extremely low frequency electromagnetic fields(ELF-EMF),primary cultured hippocampal neurons from rats were pretreated with LSPC before exposure to ELF-EMF.Survival rates,the morphology of neurons and the level of reactive oxygen species(ROS) were measured.The survival rates of hippocampal neurons pretreated with LSPC(2.5,5 μg/mL and 10 μg/mL) were 81.23%,90.18% and 93.45%,respectively,which were significantly higher than those from the model group(66.88%,P 0.05).Electromagnetic radiation increased the ROS level in neurons(the fluorescence intensity was(228.19 ± 16.28),while pretreatment with 2.5,5,10 μg/mL of LSPC reduced ROS level obviously(P 0.05).All these studies indicated pretreatment with LSPC could significantly block hippocampal neuron injury induced by extremely low frequency electromagnetic field.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第13期229-233,共5页
Food Science
基金
国家自然科学基金项目(309700309)
国家重大新药创制科技专项(2009ZX09502~015
2012ZX09501~001)
国家博士后基金项目(20100471379)
江苏大学人才基金项目(05JDG013
128300289)
关键词
极低频电磁场
海马神经元
莲房原花青素
活性氧
extremely low frequency electromagnetic field
hippocampal neuron
procyanidins from lotus seedpod(LSPC)
reactive oxygen species(ROS)