猪股骨不同部位胶原蛋白水解产物降血压作用

Antihypertensive Effects of Collagen Peptide from Hydrolysates of Different Parts of Swine Femur

以猪全股骨、股骨干、股骨头胶原蛋白水解产物为样品,研究其对自发性高血压大鼠(SHR)血压的影响。SHR适应性喂养1周后随机分为11组,每组8只:阴性对照组灌胃蒸馏水,3种样品各低、中、高3个剂量组,分别按20.0、40.0、60.0mg/kg的剂量灌胃相应的胶原蛋白水解产物,阳性对照组灌胃卡托普利,剂量为10.0mg/kg,灌胃后每2h测定各组SHR尾动脉收缩压。结果表明:股骨头组的低剂量组、全股骨和股骨干样品组对SHR没有显著的降压效果,股骨头中、高剂量组能显著降低SHR的血压。采用紫外分光光度法检测样品的ACE抑制率,全股骨和股骨头样品的ACE半抑制质量浓度(IC50)分别为3.546、2.045mg/mL。可见股骨胶原蛋白水解产物在体外能有效抑制ACE的活性,但只有股骨头胶原蛋白水解产物在较高质量浓度条件下能有效降低SHR血压。

Collagen hydrolysates prepared from the whole swine femur,femoral shaft and femoral head were tested for their antihypertensive effects in spontaneous hypertension rats（SHRs）.SHRs were fed adaptively for 7 days and then randomly divided into 11 groups of 8 each： negative group was treated with distilled water;each hydrolysate had 3 dose groups（lowdose group,middle-dose group and high-dose group）,which were treated with 20.0,40.0 mg/kg and 60.0 mg/kg.ig collagen hydrolysates respectively;and positive group was treated with 10.0 mg/kg.ig captopril.The rats’ systolic pressure was measured every 2 hours.The results indicated that hydrolysates from the whole swine femur and femoral shaft and the low dose group of femoral head hydrolysate had no significant antihypertensive effect;the middle-dose and high-dose groups of femoral head significantly lowered the blood pressure in SHRs.Spectrophotometry were used to detect the ACE inhibition rate of the different hydrolysates.The hydrolysates of whole femur and femoral head had values of IC50against ACE of 3.546 mg/mL and 2.045 mg/mL,respectively.Thus these three hydrolysates have potent ACE inhibitory activity in vitro,but only a certain concentration of femoral head hydrolysates can effectively reduce the systolic pressure in SHRs.