针刺阻抑幼鼠白内障形成及晶体谷胱甘肽耗竭的研究

Inhibitory Effects of Acupuncture on Cataractogenesis and Glutathione (GSH)-Depleted of Lens in New Born Rat

本文观察了针刺对白内障形成和晶状体内谷胱甘肽 (GSH、GSSG)耗竭的阻抑作用 ,并为临床针刺防治早期白内障提供科学的依据。采用L Buthionine (S·R) Sulfoximine(BSO)药物诱发新生幼鼠晶状体GSH的耗竭 ,形成双侧白内障。并随机分成空白对照组、药物致障组、针刺治疗组 ,同步进行研究及探索其新生鼠白内障的形成与晶状体GSH、GSSG水平的相关性。结果 :新生鼠诞生后第 9天加上第 1 7天全部致障率综合统计数据提示 ,空白对照组的致障率为 0 % (0 / 1 8) ;药物致障组的致障率为 88 89% (1 6 / 1 8) ,其第 9天的GSH含量水平比空白对照组显著下降 (P <0 0 0 1 ) ;针刺治疗组的致障率为 2 1 4% (3/ 1 4) ,其中第 9天新生鼠的致障率为 0 % (0 / 5 ) ,第 1 7天的致障率为 33 3% (3/ 9) ,与药物致障组比较有显著差异 (P <0 0 5 ) ,其晶体内的GSH含量 ,第 1 7天也比第 9天极度升高 (P <0 0 0 1 ) ,同时也与空白对照组及药物致障组有显著差异 (P <0 0 5 )。其氧化型谷胱甘肽GSSG含量 ,除空白组第 1 7天比第 9天有显著差异 (P <0 0 5 )外 ,另外两组并无显著差异。说明针刺确实可以提高晶体内GSH含量 。

The purpose of this study was to examine the inhibitory effects of acupuncture on cataractogenesis and glutathione(GSH)-depleted of lens in new born rats with buthionine sulfoximine (BSO)Methods:The glutathione synthesis inhibitor,BSO (3 mmol/kg) plus solvent were intraperitoneally injected into new born rats for three consecutive days beginning at postnatal day 2These GSH depleted rats develop cataractsHalf of the BSO-injected animals received a daily treatment of acupuncture for 30 min before the injection of BSO plus solvent while all of untreated age-matched new born rats(control group) received a daily injection of the solventThe incidence of cataracts was observed on day 9 or day 17 while the eyes of the new born rats had openedBoth reduced glutathione(GSH) and oxidized glutathione(GSSG) levels in lens were measured Results:The total animals be killed on either day 9 or day 17.The incidence of cataracts in all animals treated with BSO plus solvent was 8889%(16/18)The lenticular GSH levels at day 9 were significantly lower(compared to control group), P<0001The lenticular GSH levels at day 17 were higher than that at day 9, P<0001The incidence of cataracts in all animals co-treated with acupuncture was 214%(3/14);it was only 0%(0/5)at day 9, the incidence of cataracts was significantly decreased, P<005 The lenticular GSH levels at day 17 were significantly higher than that of BSO group or control group(P<005) and than GSH levels at day 9 (P<0001)The incidence of cataracts in all animals of non -treatment control group was 0%(0/18)Conclusion:The inhibitory effect of acupuncture treatment on cataracts formation in this study could be due to acupuncture's free radical scavenging activity or due to its stimulatory effect on glutathione production