清热止泻方治疗急性放射性肠炎的作用机制

Activity and Mechanism of Qingre zhixiefang in Treating Acute Radiation Enteritis in Rats

目的探讨清热止泻方在治疗大鼠急性放射性肠炎(ARE)中的作用机制。方法将48只成年健康Wistar雄性大鼠随机分为6组:空白对照组,模型组(给予0.9%氯化钠溶液),阳性对照组(给予地塞米松1.425 mg.kg-1),清热止泻方高、中、低剂量组(给予清热止泻方煎剂16.0,12.0和8.0 g.kg-1),每组8只。各组大鼠于造模成功后第2天开始保留灌肠给予干预,隔日一次灌肠1周,3 d后麻醉采血测定血清中丙二醛(MDA)和超氧化物歧化酶(SOD)活性,取放射区域直肠组织进行组织学观察。结果组织学变化证实阳性对照组、清热止泻方高、中剂量组均可有效治疗模型大鼠ARE;阳性对照组和清热止泻方中剂量组血清中MDA水平高于空白对照组(P>0.05),但明显低于模型和清热止泻方低剂量组(P<0.01),而SOD酶活性水平略低于空白对照组(P>0.05),但明显高于模型和清热止泻方低剂量组(P<0.01)。结论清热止泻汤可以有效降低体内MDA水平和维持SOD活性的正常水平,可能是清热止泻方治疗ARE作用机制中的重要环节之一。

Objective To evaluate the possible role of qingre zhixiefang in treating acute radiation enteritis （ ARE ） in rats. Methods Forty eight Wistar male rats were randomly divided into 6 groups （8 rats in each group） , including the blank control, model control （nowaal saline ）, positive control （dexamethasone 1. 425 mg· kg^-1）, qingre zhixiefang at high （ 16.0 g · kg^-1） , medium （ 12.0 g· kg^-1 ） and low dose （8.0 g· kg^-1 ） groups. One day after abdominal irradiation, the rats were administrated rectally with placebo or drugs every other day for one week. The morphologic changes （ HE stain） and the sermn activity of malonaldehyde （ MDA ） and superoxide dismutase （SOD） were evaluated on the third day after abdominal irradiation. The tissue within the radiation area was taken out for histological test. Results It was shown that the positive control and qingre zhixiefang at high and middle doses were effective on ARE fl＇om the histopathologic evaluation （ HE stain ）. The activity of serum MDA in the positive control and the middle dose groups was significantly lower than that in the model control and the low dose group （P〈0.01）, but higher than the blank control group （P〉0.05）. The activity of serum SOD was significantly higher than the model group and the low dose group （ P〈0.01 ）, but lower than the blank control group （ P〉0.05 ）. Conclusion Reduction of MDA level and sustaining serum SOD activity may provide explanations to the therapeutic effect of qingre zhixieJhng on ARE.