人参皂苷Rg3对乳腺癌MCF-7细胞增殖和侵袭的影响

Effect of Ginsenoside Rg3 on Cell proliferation and Invasion of Breast Cancer MCF-7 Cell Line

目的观察人参皂苷Rg3对雌激素受体阳性的乳腺癌细胞MCF-7增殖和侵袭的影响,并探讨其可能的作用机制。方法采用MTT法检测细胞的增殖能力,流式细胞仪分析细胞周期分布以及凋亡比率,通过Transwell小室观察细胞侵袭力,RT-PCR法检测细胞中的MMP-9 mRNA的表达。结果与对照组相比,人参皂苷Rg3能显著抑制MCF-7细胞的增殖;G0/G1期及S期细胞比例减少,而G2/M期细胞比例显著增加;同时细胞凋亡比率亦明显提升,而细胞侵袭指数降低,且呈现良好的剂量、时间依赖性。同时人参皂苷Rg3还能显著抑制细胞中MMP-9 mRNA的表达水平(P<0.05)。结论人参皂苷Rg3能抑制MCF-7细胞的增殖和侵袭,其作用机制可能与其能降低MMP-9基因的表达有关。

OBJECTIVE To investigate the effect of ginsenoside Rg3 on cell proliferation and invasion of estrogen receptor（ER） positive breast cancer MCF-7 cell line and the potential mechanism.METHODS The proliferation of MCF-7 cell was detected by MTT assay.Cell cycle and apoptosis rate were analyzed by flow cytometry.The effect on cell invasion was assayed by using the transwell methods.The expression of MMP-9 mRNA in MCF-7 cell was analyzed with the RT-PCR.RESULTS Compared with the control group,ginsenoside Rg3 could significantly inhibit the proliferation of MCF-7 breast cancer cells.The cell cycle of MCF-7 was also changed after treated with the ginsenoside Rg3.The percentage of cells in G0/G1 and S phase were decreased,whereas the percentage of cells in G2/M was increased significantly.ginsenoside Rg3 could also induce the MCF-7 cell apoptosis and decrease the index of cell invasion on a time and dose-depended manner.Meanwhile,ginsenoside Rg3 could significantly suppress the expression of MMP-9 mRNA on MCF-7 cell（P0.05）.CONCLUSION ginsenoside Rg3 could inhibit the proliferation and invasion of breast cancer MCF-7 cell line,the underlying mechanism may be related with the inhibitory effect of ginsenoside Rg3 on MMP-9 mRNA expression in MCF-7 cell.