灯盏花素注射液促进周围神经损伤修复的研究

Study on efficacy of breviscapine to improve peripheral nerve repair

目的观察灯盏花素注射液对周围神经损伤的修复作用,为其临床应用提供科研依据及参考。方法 SD大鼠18只,在其右侧梨状肌下缘2 cm处制15 mm坐骨神经缺损,随即均分为3组:自体移植组(坐骨神经切取后调换方向缝合);实验组(大鼠坐骨神经脱细胞支架移植+肌肉注射灯盏花素注射液1 mg.kg-1.d-1);支架组(SD大鼠坐骨神经脱细胞支架移植修复);术后12周,通过坐骨神经功能指数测定、神经组织学检测及腓肠肌湿质量恢复率评价坐骨神经修复效果。结果实验组修复神经有效截面积、神经纤维密度、纤维直径、纤维数目及髓鞘厚度均优于支架组,与自体移植组相似。同时,实验组神经S-100蛋白表达量、足底皮肤单位面积内触觉小体均明显高于支架组。结论灯盏花素注射液对周围神经的修复有明显促进作用,可用于周围神经损伤的辅助治疗。

Objective To investigate the function of breviscapine to improve peripheral nerve repair,and to provide scientific basis and reference for its uses.Methods Eighteen SD rats were obtained and made a 15mm-long nerve defect on the right sciatic nerve 2cm under lower edge of piriformis,and then were randomly divided into 3 groups： Autografts group（the nerve was cut and change direction to bridge）;Experimental group（the nerve was removed and was bridged by acellular rat sciatic nerve,and then injected with breviscapine by 1mg / Kg / d）;Nerve scaffold group（the nerve was removed and was bridged by acellular rat sciatic nerve）.12 weeks after the operation,repair effect was evaluated by sciatic verve function index（SFI）,histology detection and gastrocnemius wet weight recovery rate.Results The quantity and diameter of opaque nerve fibers,the density and neural sheath thickness in experimental group were all better than those in nerve scaffold group,and similar to those in autografts group.Meanwhile,the expression of S-100 protein in the repaired nerve and the amount of tactile corpuscle in average area in the experimental group were obviously higher than those in nerve scaffold group.Conclusion Breviscapine injection is able to improve the efficacy to repair peripheral nerve damage,and can be used in adjuvant therapy in peripheral nerve damage.