温和灸对高脂血症大鼠主动脉形态及内皮炎症反应的影响

Effect of warming Moxibustion on Aortic morphology and inflammatory response of Vascular Endothelial cells in rats with hyperlipemia

目的观察温和灸对慢性高血脂状态大鼠血管内皮形态学改变及炎症反应的影响,探讨艾灸保护血管内皮的作用机制。方法采用脂肪乳剂灌胃大鼠4周(每日10 mg/kg)造成高脂血症模型。将48只SD雄性成年大鼠随机分为正常组、模型对照组、温和灸组、药物治疗组,共4组。温和灸组予10 min×1次/天的清艾条温和灸,药物治疗组用洛伐他汀(每日10 mg/kg)混悬液灌胃,治疗4周。治疗结束取血清,生化比色法测定血清胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL-c),双抗体夹心法测定血清白细胞介素-6(IL-6)、白细胞介素-8(IL-8)、肿瘤坏死因子-α(TNF-α)。分离并剪取胸主动脉,应用HE染色观察血管内皮形态,免疫组织化学方法检测主动脉血管细胞粘附分子-1(VCAM-1)的表达。结果①温和灸组对血脂具有良好的调整作用,降低TC、LDL-c的效应均优于药物组(P<0.01)。②温和灸组血清IL-6、IL-8、TNF-α水平以及主动脉VCAM-1表达较模型组显著降低(P<0.01),与药物治疗组比较差异无显著性(P>0.05)。③温和灸组血管内皮损伤较模型对照组明显改善,内膜、中膜结构较规整。结论温和灸可通过调节血脂、抑制炎症反应,有效保护血管内皮细胞,对动脉粥样硬化早期病变具有良好的防治作用。

Objective To observe the effect of warming moxibustion on vascular endothelial morphology and inflammatory response in rats with chronic hyperlipemia and to investigate the mechanism of moxibustion protecting endothelial cells.Methods The rats were fed with fat emulsion for four weeks,causing hyperlipemia models（10mg /kg daily）.Totally 48 male adult SD rats were randomly divided into 4 groups： normal control group,hyperlipemia model group,warming moxibustion group,drug treatment group.The rats of warming moxibustion group were treated with Qing Ai 10min × 1 time / day,the rats of drug treatment group were treated with suspension of lovastatin（10mg / kg daily）,treatment lasted for 4 weeks.After treatment,Chromatography method was used for testing serum total cholesterol（TC）,triglyceride（TG）,low density lipoprotein（LDL-c）,double antibody sandwich method for measuring serum interleukin-6（IL-6） interleukin-8（IL-8）,tumor necrosis factor-a（TNF-a）,immunohistochemistry was used to observe the expression of vascular cell adherence molecules-1（VCAM-1） in thoracic aorta.Results ①Warming moxibustion group had a good adjustment of blood lipid.The level of serum TC,LDL-c in warming moxibustion group was significantly less than that in drug treatment group（P 0.01）.②Compared with hyperlipemia model group,the rats of warming moxibustion group serum IL-6,IL-8,TNF-a level and the expression of VCAM-1 in thoracic aorta was significantly decreased（P 0.01）,but there was no significant difference between warming moxibustion group and drug treatment group（P 0.05）.③Vascular endothelial injury of warming improved significantly compared with hyperlipemia model group,there were regular structures in the intima and media of moxibustion group.Conclusion Warming moxibustion can protect vascular endothelial cells effectively by regulating blood lipid and inhibiting the inflammatory response for preventing and treating early lesions of atherosclerosis.