多杀性巴氏杆菌分离鉴定及序列分析

Isolation,Identification and Sequence Analysis of Pasteurella multocida

旨在了解新疆地区多杀性巴氏杆菌主要流行株的特性。通过病原的分离纯化、PCR扩增及测序,分析8株多杀性巴氏杆菌的血清型,16SrDNA基因、ompH基因和ompA基因的差异。结果表明,3株羊源、2株牛源和1株禽源分离株为荚膜A型,1株牦牛源分离株为荚膜B型,1株标准株为荚膜E型,均具有很强的致病性。分离株与GenBank公布的代表株的16SrDNA基因、ompH及ompA基因同源性分别为93%～100%、93%～100%、98%～100%。ompH基因的ORF氨基酸C端最后10个氨基酸变化较大,分离到5株ompA基因的ORF氨基酸序列N端多出6个氨基酸(M-K-R-I-I-Q)替代原先的起始位置。可见:新疆主要流行株为强致病性的荚膜A型,且有出现变异趋势。

In order to understand the characteristics of the major epidemic strain of Pasteurella multocida in Xinjiang,the differences among serotype,16S rDNA,ompH and ompA gene sequence from eight Pasteurella multocida were examined and analyzed with isolation and PCR methods.The result showed that isolates from three sheep sources,two cattle sources and an avian source were capsular type A.A yak source was capsular type B.A standard strain was capsular type E.All of them were strong pathogenicity.The homology of 16S rDNA,ompH and ompA from eight Pasteurella multocida were 93%-100%,93%-100% and 98%-100% respectively.The last 10 amino acids in C-terminal of ompH gene ORF were different.The N-terminal was added six amino acids（M-K-R-I-I-Q）,which replaced the original starting sequences in ORF of ompA gene from the recently five isolates.The epidemic strains were strong pathogenic and capsular type A in Xinjiang.Based on these data,we speculate that the mutants would appear.