Ca^(2＋)诱导损伤线粒体超微结构的原子力显微镜研究

Ultrastructure of Injured Mitochondria Induced with Ca^(2+) Ions Observed Using Atomic Force Microscopy

采用Ca2+急性诱导构建线粒体损伤模型,分别通过透射电子显微镜和原子力显微镜观测线粒体内部结构和表面形貌的变化,用以探究线粒体损伤过程中结构变化的规律性。结果显示,线粒体在Ca2+诱导下的损伤过程依照内部结构的变化规律可分为前后2个阶段:前期以嵴有序结构的破坏为特征,线粒体内部特异性结构逐步被破坏,无明显肿胀趋势;后期以线粒体嵴间隙膨胀、嵴囊泡化为特征,线粒体迅速膨胀并向球形变化,线粒体的最终裂解应存在临界条件,决定于线粒体外膜的张力特性。

The mode of mitochondria injury induced by calcium ions（Ca2＋） was established firstly.Then,Transmission Electron Microscopy（TEM） and Atomic Force Microscopy（AFM） were used to study the internal structure and topology of injured mitochondria respectively in the Ca2＋ inducing process.The results demonstrated that changes in the internal structure of the injured mitochondria could be divided into two stages,i.e early and later stages.The specific internal structures collapsed gradually in the early stage,which was marked by the orderly distribution of mitochondrial cristae.And the mitochondria swelling rapidly became a spheroid in the later stage,which was a result of the intervals of mitochondrial cristae expanding to become vacuoles.The destruction of swelling mitochondria should have a critical condition witch is defined by tension of outer membrane of mitochondria.