新疆矮化密植枣园红枣叶斑病病原鉴定

Identification of the pathogenic fungus causing leaf spot of Zizyphus jujuba in dwarf and close-planting orchards in Xinjiang

从新疆红枣主要种植区巴音郭楞蒙古自治州、阿克苏、喀什和和田地区的46个枣园中采集叶斑病典型病样161份,分离获得了161个分离物,其中链格孢菌Alternaria 141个,占总分离物的87.58%,镰刀菌Fusarium 12个,占7.45%。致病性测定后,采用形态学观察结合18S rDNA-ITS、EF-1α和β-tubulin 3个基因序列分析,鉴定新疆矮化密植枣园红枣叶斑病的主要病原菌为A.alter-nata(Fries)Keissle。

A total of 161 samples of leaf spot disease were collected from 46 jujube orchards of dwarf and close-planting in Bayingolin Mongolia Autonomous Prefecture,Akzo,Kashi and Hotan Prefecture in Xinjiang and 161 isolates were isolated.Among them,there were 141 Alternaria isolates,accounting for 87.58% of the total,which was the main pathogen for leaf spot of Zizyphus jujuba in Xinjiang and there were 12 Fusarium isolates,accounting for 7.45%.According to the morphological features of culture and the ITS sequence,EF-1α and β-tubulin gene sequence analysis,these Alternaria isolates were identified as A.alternata（Fries） Keissle,which was main pathogen of leaf spot of Z.jujuba.