清热散瘀解毒方对发热大鼠血清TNF-α、IL-1β、COX-2含量的影响

Influence of Qinresanyujiedu Decoction on content of rat's serum TNF-α、IL-1β、COX-2

目的观察清热散瘀解毒方对2,4-二硝基苯酚诱导大鼠发热模型造模后2 h体温以及血清中TNF-α、IL-1β、COX-2等内生性致热原含量变化,探讨清热散瘀解毒方治疗癌性发热的作用机制。方法将实验大鼠分为空白组6只,模型组、中药组、西药组各12只。在模型组、中药组、西药组大鼠皮下注射2,4-二硝基苯酚造成非感染性发热模型。造模后立即给各组大鼠分别灌药:空白组、模型组灌入生理盐水,中药组、西药组分别予清热散瘀解毒方水煎液与萘普生。灌药后观察和比较各组大鼠造模2 h后体温及血清中TNF-α、IL-1β、COX-2含量变化。结果中药组、西药组造模2 h后体温低于模型组(P<0.05);中药组、西药组血清中TNF-α、IL-1β、COX-2的含量低于模型组(P<0.05)。结论清热散瘀解毒方能降低2,4-二硝基苯酚诱导大鼠发热模型的体温,其作用机制与降低发热大鼠模型血清中TNF-α、IL-1β、COX-2含量相关。

Objective：To observe the influence of Qinresanyujiedu Decoction on fever in rats model＇s body temperature induced by 2,4-two nitrophenol and content changes of Endogenous pyrogen TNF-α,IL-1β,COX-2 Treatment.And to explore the thermal mechanism of Qinresanyujiedu Decoction on treating blood stasis type of cancer patients.Methods：Rats were divided into three groups：blank group,model group 6,Chinese medicine group and Western medicine group,12 rats in each.Rats in each group were poured medicine：blank group,model group filled with saline,traditional Chinese medicine group and Western medicine group.Subcutaneous was injected with 2,4-two nitrophenol to model group,traditional Chinese medicine group and Western medicine group caused non-infective fever model.And body temperature and content changes of Endogenous pyrogen TNF-α,IL1β,COX-2 after building model 2 h later were observed.Results：After building model 2h later,The temperature of Qinresanyujiedufang group was lower than model group and the difference is significantl（P0.05）.The rats serum TNF-α,IL-1β,and COX-2 was lower than the model group and the difference is obviously（P0.05）.Conclusion：Qinresanyujiedu Decoction can treatment blood stasis type of cancerous fever,its mechanism may be related to reduce the content of rat serum TNF-α,IL-1β,and COX-2.