薄荷GPPS基因的克隆与表达分析

Cloning and Expression Analysis of Gene GPPS in Mint

牻牛儿基焦磷酸合酶(GPPS Geranyl diphosphate synthase)为短链异戊烯基合酶家族成员,催化1分子的IPP与DMAPP形成GPP,为单萜提供碳骨架。实验从我国薄荷品种"738"中克隆了GPPS大小亚基cDNA全长序列,对其进行序列分析,并研究GPPS基因在薄荷根、茎、叶、花中的相对表达量。结果:GPPS大亚基cDNA序列ORF全长1131 bp,编码377个氨基酸,GPPS小亚基cDNA序列ORF全长942 bp,编码313个氨基酸。研究获得的GPPS基因所编码氨基酸与薄荷属其他种的GPPS氨基酸序列高度相似。GPPS大亚基基因在薄荷品种"738"不同组织中均有表达,幼叶中的表达量最高。

Geranyl diphosphate synthase (GPPS) is a member of short-chain prenyltransferase family, which can catalyze the formation of Geranyl diphosphate (GPP) from IPP and DMAPP, and GPP can provide carbon skeleton for monoterpene .The cDNA complete sequences containing large and small subunit of GPPS were cloned from mint variety “738” in this research.The se-quences were analyzed, and the relative expression of the gene GPPS in the root, stem, leaf and flower of mint was also studied . The results showed that GPPS large subunit cDNA complete sequences had a 1131 bp ORF encoding 377 amino acid residues, and GPPS small subunit cDNA complete sequences had a 942 bp ORF encoding 313 amino acid residues.GPPS large and small subunit protein shared high identity with other mint varieties .GPPS large subunit expression was found in different tissues of mint variety“738”, and the expression in young leaves was stronger than that in other tissues .