决定七氟醚敏感性的基因在果蝇常染色体的定位研究

Localization of a gene determining the sensitivity to sevoflurane in autosomal chromosomes of Drosophila melanogaster

目的 在染色体水平对决定果蝇七氟醚敏感性的基因进行定位。方法 将H(黑腹 )处女雌蝇与e(黑檀体 )雄蝇进行杂交产生杂合子一代 (F1)。取F1雄蝇与eF1处女雌蝇进行侧交产生子二代 (F2 )。按性别及表型分别测定三代果蝇七氟醚麻醉的ED50 值 ,比较侧交F2 中黑腹、黑檀体表型果蝇和H、e自交F2 果蝇的ED50 值 ,并作浓度 -效应曲线。结果 杂合F1ED50 与HF1ED50 相等 ,大于eF1的ED50 值。侧交F2 中 49.76 %表现为黑腹 ,5 0 .2 4%表现为黑檀体。F2 中黑腹和黑檀体ED50值大于eF2 的ED50 ,小于HF2 的ED50 值 ,且其量 -效曲线均为双峰组成 ,而H和e自交F2 的量 -效曲线均为单峰。结论 决定黑腹果蝇对七氟醚敏感性的基因位于果蝇第二号染色体。

Objective To study a gene determing the sensitivity to sevoflurane in autosomal chromosomes of Drosophila melanogaster. Methods Virgin females of wild type Drosophila melanogaster(H) were crossed with ebony(e) males to breeding F 1 hybrids ,to measure the ED 50 of e,H and F 1 for sevoflurane. Then, e virgin females were crossed with F 1 males to breed F 2 hybrids. Sevoflurane ED 50 of F 2 hybrids was measured according to body's colour. The ED 50 was measured when the fruit flies were 7 days old.A hereditary analysis was conducted to determine the localization of gene(s) in chromosome determining sensitivitiy to sevoflurane. Results The ED 50 of H was significantly higher than that of e . The ED 50 of phenotype of F 1 hybrids was similar to one of H of F 1.The number ratio of flies with black abdomen to ebony flies was about 1∶1 in F 2 hybrids which generated from crosses of F 1 hybrid males with e virgin females. The sevoflurane ED 50 of F 2 hybrids was significantly higher than that of F 2 fly of e and was significantly lower than the ED 50 of F 2 fly of H . The dose response curve of F 2 hybrids had a two peak, however, the dose response curve of F 2 fruit fly of e or H had a single peak. Conclusions The gene(s) or major gene(s) determining the sensitivity to sevoflurane is (are) located on the second chromosome of Drosophila melanogaster.