塞来昔布抑制人结肠癌细胞Caco-2细胞增殖及其分子机制

Modulating effect of celecoxib to suppress proliferation and the molecular mechanism in caco-2 of colon carcinoma cell line

目的研究塞来昔布在体外抑制人结肠癌细胞Caco-2生长增殖及其抗肿瘤的相关分子机制。方法体外培养人结肠癌细胞Caco-2,分组为正常组(无任何干预)及塞来昔布组。四甲基偶氮唑盐(MTT)法检测塞来昔布在相同浓度下,不同时间对于胃癌细胞增殖的影响,并计算半数抑制浓度(IC50)值;反转录聚合酶链式反应(RT-PCR)法检环氧化酶-2(COX-2)、基质金属蛋白酶9(MMP-9)的mRNA的表达影响。结果 MTT结果显示:相同干预浓度塞来昔布抑制人胃癌细胞增殖,其24 h,48 h,72 h的IC50分别为:99.519±10.355μmol/L、71.546±6.446μmol/L、59.622±15.999μmol/L;RT-PCR结果显示:人结肠癌细胞Caco-2正常对照组及塞来昔布干预组COX-2mRNA灰度值分别为:0.808±0.021,0.101±0.002(t=19.037,P=0.000);MMP-9 mRNA灰度值分别为:0.798±0.031,0.190±0.002(t=18.987,P=0.002)。结论塞来昔布可能通过抑制COX-2、MMP-9的mRNA的表达,从而抑制结肠癌细胞增殖。

Objective To study celecoxib in vitro human colon cancer cell growth and proliferation of Caco-2 and its anti-tumor molecular mechanisms.Methods Cultured human colon cancer Caco-2 were divided to two group（one group is the control group that has no interference,the other group is celecoxib interference group）.MTT method was used to analyse celecoxib at the same concentration,at different times for the colon cancer cell proliferation and calculate IC50 value.RT-PCR method was used to evaluate the expression of COX-2,MMP-9 mRNA.Results MTT assay showed the concentration of the same intervention celecoxib to inhibit the proliferation of human colon cancer cells,its 24 h,48 h,72h IC50 of respectively： 99.519 ± 10.355μmol / L,71.546 ± 6.446 μmol / L,59.622 ± 15.999 μmol / L.The RT-PCR results show that human colon cancer cells Caco-2 normal control group and celecoxib in the intervention group,respectively,the COX-2mRNA gray value for： 0.873 ± 0.026,0.115 ± 0.008,P 0.05.MMP-9 mRNA gray values were： 0.808 ± 0.021,0.101 ± 0.002（t = 19.037,P = 0.000）.MMP-9 mRNA were 0.798 ± 0.031,0.190 ± 0.002（t = 18.987,P = 0.002）.Conclusion Celecoxib can inhibit gastric cancer cell proliferation.Celecoxib antitumor mechanisms may by inhibiting the expression of COX-2 and MMP-9 mRNA in implementation.