猫扣带回前部内脏伤害与非伤害感受神经元自发放电特性的胞内记录

Characteristics of Spontaneous Discharges of ACG Visceral Nociceptive Neurons and Non-nociceptive Ones in Cats with Intracellular Recording

本文应用电刺激内脏大神经作为内脏痛的实验动物模型 ,通过在体玻璃微电极细胞内电位记录技术 ,观察了 2 0只猫扣带回前部 1 76个内脏大神经刺激相关神经元的自发放电特性。根据神经元对电刺激内脏大神经产生的诱发反应特性的不同 ,将其分为内脏伤害感受神经元(84 0 9% )和非伤害感受神经元 (1 5 91 % )。结果表明 ,有自发放电活动的内脏伤害感受神经元比例数明显高于内脏非伤害感受神经元 ,前者的静息电位明显低于后者 ,但是 ,两者的自发放电形式和频率无明显差异。提示扣带回前部存在内脏伤害感受神经元和内脏非伤害感受神经元 ,前者的兴奋性高于后者。

Electrically stimulating greater splanchnic nerve(GSN) was used as an experiment model of visceral pain.The spontaneous firings of 176 stimulus-relative neurons in anterior cingulate gyrus(ACG)were analyzed by means of the intracellular recording techniques of glass microelectrode in cats.According to characteristics of the evoked response to stimulating GSN,stimulus-relative neurons could be classified into visceral nociceptive neurons(VNNs,8401%)and visceral non-nociceptive neurons(NVNNs,1591%).The main findings were as follows:(1)The percentage of number of spontaneous firing neurons was significantly higher in VNNs than in NVNNs.(2)The resting membrane potential of the former was significantly lower than that of the latter.(3)Modes and frequency of spontaneous discharges of both were not obviously different. The results indicated that there existed VNNs and NVNNs in ACG,and that the excitability of the VNNs was higher than that of the NVNNs.