安吉白茶、桑叶复合功能性饮料对D-半乳糖致小鼠氧化损伤的拮抗效应研究

Antagonism Effects of a Compound Functional Beverage with Camellia sinensis L. and Morus alba L. on D-Galactose Induced Oxidative Damage in Mice

本文采用D-半乳糖建立小鼠氧化损伤(应激)模型,研究了不同剂量安吉白茶、桑叶复合功能性饮料喷雾干粉DPCB(100、200、500 mg/kg.bw)对小鼠体重、脏器指数,血清、肝脏中丙二醛(MDA)含量和谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)活性,以及对肝脏组织病理学的影响。结果表明,DPCB可使氧化损伤小鼠体重增量、胸腺指数显著升高,使肝脏、肾脏和脾脏指数也出现一定程度的升高;DPCB可有效降低氧化损伤小鼠血清和肝脏中MDA含量,最大降幅分别为21%和15%;同时有效增高氧化损伤小鼠血清和肝脏中GSH-Px、SOD的活性,且具有量效关系;此外,DPCB对肝脏组织的病理学改变有一定拮抗效应。本研究为安吉白茶、桑叶天然抗氧化功能性食品的开发奠定了基础。

The present study mainly investigated the dry powder of compound functional beverage with Camellia sinensis L.and Morus alba（DPCB） on D-galactose induced oxidative stress in mice,and determined the changes of body weight,viscera index,serum and liver MDA content,GSH-Px,SOD activities,and hepatic histopathology.The results indicated that the body weight gain,thymus index,spleen index,liver index,and kidney index in different dose groups of DPCB significantly increased,compared with the oxidative stress model group.The serum and hepatic MDA content in high dose DPCB mice decreased 21% and 15%,respectively,compared to oxidative stress mice.DPCB could increase the serum and hepatic GSH-Px and SOD activities in a dose-dependent manner.In addition,DPCB could protect the hepatic histopathologic changes.This study laid a foundation for DPCB exploitation to antioxidant functional foods.