甘蔗渣多酚的纯化及抗氧化活性研究

Purification and Antioxidant Activity of Sugarcane Pomace Polyphenols

本文利用大孔吸附树脂通过静态吸附与解吸实验研究了甘蔗渣中多酚的分离纯化工艺,并利用DPPH自由基体系、邻苯三酚法检测了甘蔗渣多酚的抗氧化活性。结果表明:NKA-9型树脂是富集纯化甘蔗多酚的最好材料;NKA-9树脂的最佳动态吸附条件为甘蔗多酚液浓度2.50 mg/mL,流速1.00 mL/min,最优洗脱条件为乙醇浓度60%,洗脱流速1.00 mL/min,洗脱体积10 BV,此条件下甘蔗多酚的收率为72.32%,纯度可达39.21%;抗氧化活性实验表明,甘蔗多酚对超氧阴离子O2-.和DPPH都具有良好的清除效果,并随多酚浓度的增加,其清除效果也相应提高;与Vc相比,甘蔗多酚对DPPH的清除效果不及Vc,但对超氧阴离子O2-.的清除效果明显高于Vc;甘蔗多酚浓度为3.00 mg/mL时,对超氧阴离子的清除率最高,达到82.3%,甘蔗多酚浓度为5.00 mg/mL时,其对DPPH溶液的清除率达80.43%。

Purification technology of polyphenols from sugarcane pomace was studied by static absorption and desorption of macroporous resin,and antioxidative activity of sugarcane polyphenols was evaluated by DPPH free radical and pyrogallol autoxidation.Results indicated that macroporous resin NKA-9 was the best material for purificating polyphenols in sugarcane pomace.The optimum absorption conditions of macroporous resin NKA-9 were concentration of feed 2.5 mg/mL and feeding rate 1.0 mL/min.The optimum desorption conditions were elute-ethanol concentration 60%,elution rate 1.0 mL/min and elute volume 10 BV.Under conditions in which the yield of sugarcane polyphenols was 72.3% and purity was 39.2%.The sugarcane polyphenols showed high scavenging activities on superoxide anion radicals and DPPH radical,and the scavenging activity of polyphenols increased with increasing polyphenol concentration.Sugarcane polyphenols showed lower scavenging activity on DPPH radical but high scavenging activity on superoxide anion radicals than vitamin C.When sugarcane polyphenols concentration was 3.0 mg/mL,its scavenging activity on superoxide anion reached the highest,being of 82.3%.Further increasing sugarcane polyphenol concentration to 5 mg/mL,however,led to the decrease of the scavenging activity on DPPH（80.43%）.