仙鹤草对2型糖尿病大鼠胰岛素抵抗及炎性因子的干预作用

Effects of Herba Agrimoniae on insulin resisitance and inflammatory factors in T2DM rats

目的观察仙鹤草提取物对2型糖尿病大鼠血糖、血脂、胰岛素抵抗及血清中炎性因子的影响。方法采用高脂饲料喂养加腹腔注射小剂量链脲佐菌素STZ的方法制备2型糖尿病动物模型,随机分为4组(n=9):模型组、吡格列酮组、仙鹤草高剂量组、仙鹤草低剂量组。灌胃给药4周后取血检测各组大鼠空腹血糖(FBG)、甘油三酯(TG)、总胆固醇(CHOL)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C),采用放射免疫法测定空腹血清胰岛素(Fins)、肿瘤坏死因子α(TNF-α)、C反应蛋白(CRP),并根据Fins,FBG计算胰岛素抵抗指数(HOMA-IR)。结果仙鹤草高剂量可显著降低大鼠FBG,FINS,HOMA-IR;可降低甘油三酯,升高高密度脂蛋白;降低血清中炎性因子TNF-α和CRP含量。结论仙鹤草提取物对2型糖尿病大鼠具有降糖调脂的治疗作用,可缓解2型糖尿病胰岛素抵抗,并推测可能与降低血清炎性因子含量有关。

Objective To investigate the effects of Herba Agrimoniae on glucose and blood lipid, insulin resistance and the levels of inflammatory factors in T2DM Rats. Methods The T2DM rats induced by high lipid diet and small dosage intraperitonealinjection of STZ,were randomly divided into four groups （ n =9 in each） . Model,Herba Agrimoniae （ high dose and low dose） and Pioglitazone were administered respectively by gavage. Four weeks later,the levels of FBG and blood lipid were detected. Fins were measured by radioactive immunoassay and accordingly insulin resistance indexes（ HOMA-IR） were figured out. TNF-α and CRP in the serum were detected by the method of radioactive immunoassay too. Results Compared with model group,the levels of FBG,Fins,HOMA-IR ,TG,were decreased,HDL was increased. The levels of TNF-α,CRP in the serum were down-regulated in high dose Herba Agrimoniae group. Conclusions Herba Agrimoniae can decrease glucose,blood lipid and improve insulin resistance in type 2 diabetic mellitus rats by decreasing the the levels of inflammatory factors of TNF-α and CRP.