角质细胞生长因子对胰腺癌细胞生物学行为的影响

Role of KGF in regulating biological behavior of pancreatic cancer cells

目的:探讨角质细胞生长因子(keratinocyte growth factor,KGF)对胰腺癌细胞生物学功能的影响.方法:运用免疫印迹和聚合酶链反应(polymerase chain reaction,PCR)检测6株胰腺癌细胞系(CaPan-2、BxPc-3、MIAPaca-2、ASPc-1、S W-1990、PA N C-2)中K G F蛋白的表达和角质细胞生长因子受体(keratinocyte growth factor-receptor,KGFR)mRNA的表达水平.并运用MTT、细胞迁移及侵袭实验检测KGF对胰腺癌细胞株(BxPC-3)增殖、迁移及侵袭的影响.结果:免疫印迹和PCR显示,KGF在BxPC-3中的表达水平明显低于其他5株细胞系,而其受体mRNA在BxPC-3细胞中的表达水平明显高于其余5株细胞系.KGF能够明显促进BxPC-3细胞的增殖(P<0.01).并且能够明显增加BxPC-3细胞的迁移及侵袭能力(P<0.01).结论:在部分胰腺癌细胞系中,KGF能够促进细胞的增殖,并能够增加其迁移及侵袭能力.

AIM：To examine the role of keratinocyte growth factor（KGF） in regulating the biological behavior of pancreatic cancer cells.METHODS：Immunoblotting（IB） and polymerase chain reaction（PCR） were used to detect the protein and mRNA levels of KGF and its receptor in six pancreatic cancer cell lines（CaPan-2,BxPc-3,MIAPaca-2,ASPc-1,SW-1990,and PANC-2）.MTT,migration and invasion assays were used to detect the role of KGF in regulating cell proliferation,migration and invasion in pancreatic cancer cell line BxPC-3.RESULTS：The protein level of KGF was lower in BxPC-3 cells than in other five pancreatic cancer cell lines,while the mRNA level of KGFR was higher in BxPC-3 cells than in other five cell lines.KGF could significantly promote BxPc-3 cell proliferation,migration and invasion（all P 0.01）.CONCLUSION：KGF can promote cell proliferation,migration and invasion in some pancreatic cancer cell lines.