细胞因子在玻璃体切除联合硅油填充术后并发性白内障患者房水中的表达

The study of cytokines in aqueous humor of complicated cataract after vitrectomy combined with silicone oil injection

目的比较玻璃体切除联合硅油填充术后并发性白内障患者与年龄相关性白内障患者房水中细胞因子的表达差异情况,探讨细胞因子与并发性白内障的相关性。设计实验研究。研究对象北京同仁医院因玻璃体切除联合硅油填充术后3个月～6年需手术治疗的并发性白内障患者(实验组)19例(19眼)及需手术治疗的年龄相关性白内障患者(对照组)11例(11眼)。方法每例受试者白内障手术中收集房水0.1～0.2 ml,通过流式细胞仪检测房水中细胞因子IL-1β、IL-2、IL-4、IL-5、IL-6、IL-10、TNF-α、IFN-γ的表达。主要指标上述8种细胞因子的表达量。结果实验组中IL-6的表达量(199635.64±28156.5 fg/ml)及IL-10的表达量(273.57±206.7 fg/ml)均明显高于正常对照组(3340.96±1970.36 fg/ml)及(117.45±64.77 fg/ml)(P均<0.05)。其余6种细胞因子表达两组间差异则无统计学意义。结论玻璃体切除联合硅油填充术后并发性白内障患者房水中细胞因子IL-6、IL-10含量较高,可能与并发性白内障的形成或加速有关。

Objective To compare the concentrations of cytokines in aqueous humor of age-related cataract eyes and complicated cataract eyes after vitrectomy combined with silicone oil injection, and to investigate the relationship of cytokine concentrations with complicated cataract resulted from vitrectomy. Design Experimental study. Participants 19 eyes of complicated cataracts after vitrec- tomy combined with silicone oil injection and 11 eyes of age-related cataracts were enrolled into experiment group and control group, respectively. Method The cataract surgeries were performed 3 months to 6 years after vitrectomy combined with silicone oil injection. Aqueous humor of 0.1-0.2 nd was collected tbr each patient during cataract surgery. The concentrations of cytokines （IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, TNF-α, IFN-γ） in aqueous humor were measured with flow cytometry. Main Outcome Measures The concentrations of cytokines. Result The concentrations of IL-6 and IL-10 in the experiment group is 199635.64±28156.5 fg/ml and 273.57±206.7 fg/ml, respectively, both significantly higher than the concentrations in the control group （3340.96±1970.36 fg/ml and 117.45±64.77 fg/ml, respectively）. For those two cytokines, there is statistic difference between the experiment group and the control group （P〈0.05）. There is no statistic difference between the experiment group and the control group for the other six cytokines. Conclusion Based on the study of the limited samples, the concentrations of IL-6 and IL-IO in aqueous humor of complicated cataract eyes after vitrectomy combined with silicone oil injection is statistically higher than in normal controls. The IL-6 and IL-IO may result to or accelerate the occurrence of complicated cataract. （Ophthalmol CHN, 2013, 22： 269-272）