两种方法检测乙型肝炎患者血清HBV-DNA的应用价值

PCR-EB qualitative analysis versus amp-lisensor quantitative assay for detection of HBV-DNA in serum in patients with hepatitis B

目的:观察两种不同方法检测乙型肝炎患者血清中的乙型肝炎病毒(hepatitis B virus,HBV)-DNA的应用价值比较.方法:分别使用PCR-EB定性及Amp-lisensor定量法对HBV患者,乙型肝炎表面抗原(hepatitis B virus surface antigen,HBsAg)与抗-HBs同时阳性、HBsAg阳性、抗-HBs阳性、HBsAg与抗-HBs同时阴性进行血清HBV-DNA检查,观察2组患者阳性检测率.结果:B组(HBsAg阳性)患者HBSAg(S/N)值高于A组(P<0.05),C组抗-HBs值高于A组(HBsAg与抗-HBS同时阳性)(P<0.05),Amp-lisensor定量检测下,患者HBV-DNA阳性率明显高于PCR-EB定性组(P<0.05).Amp-lisensor定量检测中,C组(抗-HBS阳性)患者的阳性检测率最低(P<0.05),乙型肝炎患者血清中H B V-D N A对数值均有明显的上升,与E组(乙型肝炎两对半全阴性)比较均有统计学意义(P<0.05).A组与B组HBV-DNA对数值明显高于C组与D组(HBsAg与抗-HBs同时阴性)(P<0.05),A组与B组HBV-DNA对数值比较差异无统计学意义(P>0.05).结论:Amp-lisensor定量法检测血清HBV-DNA的灵敏度较高,在抗-HBs阳性患者中仍可以检测到HBV-DNA.

AIM： To compare the application value of PCR- EB qualitative analysis versus amp-lisensor quantitative assay in the detection of HBV-DNA in serum in patients with hepatitis B. METHODS： PCR-EB qualitative analysis or amp- lisensor quantitative assay was used to detect serum HBV-DNA levels in patients with hepatitis B who were positive for both HBsAg and anti- HBs （group A）, those positive for HBsAg alone （group B）, those positive for anti-HBs alone （group C）, or those negative for both HBsAg and anti- HBs （group D）. The detection rate of serum HBV- DNA was compared between the two groups.RESULTS： HBsAg （S/N） value was signifi- cantly higher in group than in group A （P 〈 0.05）, and anti-HBs value was significantly higher in group C than in group A （P 〈 0.05）. The detec- tion rate of serum HBV-DNA by Amp-lisensor quantitative assay was significantly higher than that by PCR-EB qualitative analysis （P 〈 0.05）. For amp-lisensor quantitative assay, the positive rate of serum HBV-DNA was lowest in patients of group C （P 〈 0.05）. Serum HBV-DNA rose significantly in patients with hepatitis B. HBV- DNA levels were significantly higher in groups A and B than in groups C and D （all P 〈 0.05）, but showed no significant differences between groups A and B （P 〉 0.05）. CONCLUSION： Amp-lisensor quantitative assay has a high sensitivity in the detection of serum HBV-DNA and is even able to detect HBV-DNA in patients who are positive for anti-HBs.