摘要
目的:检测脂多糖(LPS)刺激对体外培养人牙周膜细胞分泌炎症趋化因子IL-8和MCP-1改变的影响。方法:组织块法原代培养人牙周膜细胞,MTT法观察不同浓度LPS对其增殖的影响;取第4代牙周膜细胞在10μg/mL LPS刺激条件下进行培养,分别于培养后4、8、12、24 h用酶联免疫吸附(ELISA)法检测牙周膜细胞培养上清中IL-8和MCP-1的含量;结果采用单因素方差进行统计分析。结果:10μg/mL LPS刺激可使牙周膜细胞的增殖速度略减低,刺激后24 h内细胞形态和数量未见明显变化。ELISA结果显示LPS刺激后牙周膜细胞在10μg/mL培养上清中IL-8和MCP-1含量均明显高于对照组(P<0.05);且两种蛋白含量均随培养时间延长而增加(P<0.05),呈时间依赖性。结论:10μg/mL LPS刺激可使牙周膜细胞培养上清中IL-8和MCP-1含量呈时间依赖性增加,提示牙周膜细胞具有免疫调节功能。
AIM： To investigate the effects of LPS stimulation on the expression of IL-8 and MCP-1 of human periodontal ligament cells （HPDLCs） cultured in vitro. METHODS： HPDLCs were cultured and identified. MTI7 method was used to exam the viability of the cells stimulated by LPS at different concentrations. Enzyme linked immunosorbent assay （ELISA） was used to detect IL-8 and MCP-1 concentration in the cell culture supernatant after stimulation by 10 Ixg/mL of LPS for 4 h, 8 h, 12 h and 24 h respectively. RESULTS ： Stimulation of LPS at 10 Izg/mL within 24 h showed no significant effects on the morphology and proliferation of HPDLCs. ELISA revealed that the concentration of IL-8 and MCP-1 increased from 4 h to 24 h in a time dependent manner after LPS stimulation （vs the corresponding negative controls P 〈 0.05）. CONCLUSION ： LPS can stimulate the production of IL-8 and MCP-1 of HPDLCs and HPDLCs have the potential of immuno activity.
出处
《牙体牙髓牙周病学杂志》
CAS
北大核心
2013年第8期485-488,共4页
Chinese Journal of Conservative Dentistry
