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MicroRNA-21参与调控金华猪肝脏生长发育 被引量：4

MicroRNA-21 Participates in the Regulation of Liver Development in Jinhua Pig
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摘要 microRNAs是一种小分子非编码RNA,广泛参与细胞的生长发育、分化和凋亡等生物学过程.microRNA-21(miR-21)是一个在实体肿瘤中高表达的miRNA,可促进癌细胞增殖.为了研究miR-21在金华猪生长过程中的作用,本试验选取了金华猪80日龄胚胎的心、肝、脾、肺、肾、小肠、胰脏、皮脂组织,进行miR-21组织表达谱研究,结果发现miR-21在肝脏中的表达最高.进一步以肝脏为对象,研究miR-21在金华猪不同生长阶段中的表达,结果显示胚胎80日龄的miR-21表达量最高(P<0.01),其次是胚胎期的60日龄和105日龄,出生后1至120日龄miR-21的表达量均维持在较低的水平且相互之间差异不显著(P>0.05).利用KEGG软件对miR-21预测的靶基因进行通路归类分析表明,miR-21参与细胞代谢、生长等过程,从中选择2个抑制细胞增殖的靶基因,增强子结合蛋白(CCAAT/enhancer binding protein,Cebpa)和原肌球蛋白1基因(tropomyosin 1,Tpm1),qRT-PCR检测二者在不同生长阶段的mRNA表达量,结果其表达趋势与miR-21相反,其中胚胎期80 d表达量最低(P<0.05).综上表明,miR-21可能具有促进胚胎肝脏生长的作用.
作者 李艳华 马义涛 周辉云 王颖 徐宁迎
机构地区 浙江大学动物科学学院
出处 《中国生物化学与分子生物学报》 CAS CSCD 北大核心 2013年第8期791-795,共5页 Chinese Journal of Biochemistry and Molecular Biology
基金 国家自然科学基金项目(No.31000997 No.31272424) 浙江省重点科技创新团队项目(No.2010R50027)~~
关键词 MICRORNA-21 金华猪 肝脏
分类号 Q71 [生物学—分子生物学]
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参考文献21

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二级参考文献39

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中国生物化学与分子生物学报
2013年 第8期

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