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肺鳞癌组织Hsa-mir-182差异表达的研究 被引量：5

Different expression of Hsa-mir-182 in lung squamous cell carcinoma tissues
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摘要 目的:寻找肺鳞状细胞癌组织中与正常组织中差异表达的微小RNA(miRNA),并对其进行生物学功能的预测及验证。方法:应用芯片技术筛选30例肺鳞癌组织中与正常组织中差异表达的miRNA。选取差异表达的mir-182家族进行定量PCR验证,通过生物信息学网站预测其靶基因,并在体外进行荧光素酶报告基因验证。结果:Hsa-mir-182家族在肺鳞癌组织中表达丰度较正常组织明显升高。生物信息学网站预测发现,RASA1可能是mir-182的靶基因。体外过表达mir-182后,发现RASA1表达量显著下降,验证了RASA1确实为mir-182的靶基因。结论:Hsa-mir-182家族在肺鳞癌组织中显著高表达,而且RASA1基因系mir-182的靶基因。 OBJECTIVE：To detect the different expression of microRNA （miRNA） in lung squamous cell cancer and to predict and validate their biological function. MEI^Ol~：Microarray was used to discover the special miRNA which was differentially ex- pressed between lung squamous cell cancer tissues and normal lung tissues. The mir-182 family which was significantly upregulated in lung squamous cell cancer tissues were tested through qualitative PCR. The target genes were predicted by public algorithms and con- firmed in v/vo. RESULTS： Hsa-mir-182 family was highly expressed in lung squamous cell carcinoma. After mir-182 being overex- pressed,the expression of RASA1 was significantly down regulated, which further confirmed that RASA1 was the target gene of mir-182. CONCLUSIONS：Hsa-rnir-182 family is significantly high expressed in lung squamous cell cancer. Mir-182 downregulates RASA1 and it may play an important role in lung squamous cell cancer.
作者 徐彪 夏伟 邵宁生 朱彦君
机构地区 中国人民解放军空军总医院胸外科 军事医学科学院基础医学研究所
出处 《中华肿瘤防治杂志》 CAS 北大核心 2013年第16期1225-1228,共4页 Chinese Journal of Cancer Prevention and Treatment
关键词 MICRORNA Hsa-mir-182 肺鳞癌 基因表达 microRNA Hsa-mir-182 lung squamous cell cancer gene expression
分类号 R734.2 [医药卫生—肿瘤]
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参考文献13

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同被引文献39

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  • 7Guo H, Ingolia NT, Weissman JS, et al. Mammalian mieroRNAs pre- dominantly act to decrease larget mRNA levels [ J ]. Nature, 2010, 466 ( 7308 ) : 835 - 840.
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  • 10Cho WC, Chow AS, Au JS. Restoration of tumor suppressor has - miR - 14-5 inhibits cancer cell growth in lung adenocarcinoma patients with epidermal growth factor receptor mutation [ J ]. Eur J Cancer, 2009, 45:2197 - 2206.

引证文献5

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中华肿瘤防治杂志
2013年 第16期

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