原发性乳房外Paget病组织中钠氢交换子调节因子1和β联蛋白的表达和意义

Expressions of N HERF1 and β-catenin in primary extramammary Paget＇s disease tissue and their significance

目的探讨钠氢交换子调节因子1（NHERF1）和β联蛋白在原发性乳房外Paget病组织中的表达及其意义。方法应用免疫组化方法，检测18例原位乳房外Paget病、22例侵袭性乳房外Paget病石蜡组织切片中NHERF1和β联蛋白的表达情况。结果NHERF1在22例侵袭性乳房外Paget病中18例（81．82％）为高表达，18例原位乳房外Paget病中7例（38．89％）高表达，两组差异有统计学意义（χ2=7．78，P〈0．01）；β联蛋白在侵袭性乳房外Paget病中有0例胞膜高表达和18例（81．82％）胞质或核高表达，原位乳房外Paget病中分别为6例（33．33％）和8例（44．44％），两组差异均有统计学意义（χ2值分别为8．63和6．08，P值分别〈0．01和〈0．05）。原发性乳房外Paget病组织中NHERF1的表达与B联蛋白胞膜表达呈负相关（ρ=-0．488，P〈0．01），与β联蛋白胞质或核表达呈正相关（ρ=0．623，P〈0．01）；β联蛋白胞膜表达与胞质或核表达呈负相关（ρ=-0．572，P〈0．01）。结论乳房外Paget病组织中NHERF1和β联蛋白表达异常，并可能与原发性乳房外Paget病的发生发展及侵袭有关。

Objective To investigate the expressions of Na＋/H＋ exchanger regulatory factor 1 （NHERF1） and β-catenin in extramammary Paget＇s disease tissue as well as their significance. Methods Immunohistochemistry was performed to detect the protein expressions of NHERF1 and β-catenin in paraffin-embeded tissue samples from 18 patients with in situ and 22 patients with invasive extramammary Paget＇s disease. Results There was a high expression of NHERF1 protein in 18 （81.82%） invasive and 7 （38.89%） in situ extramammary Paget＇s disease samples （χ2 = 7.78, P 〈 0.01）. Statistical differences were observed in the membrane expression rate and cytoplasmic or nuclear expression rate of β-catenin between invasive and in situ extramammary Paget＇s disease tissue samples （0 （0/22） vs. 33.33% （6/18）, χ2 = 8.63, P 〈 0.01; 81.82% （18/22） vs. 44.44% （8/18）,χ2 = 6.08, P 〈 0.05）. In extramammary Paget＇s disease in situ tissue samples, the expression of NHERF1 was negatively correlated with the cytomembrane expression of β-catenin （ρ = -0.488, P 〈 0.01 ）, but positively correlated with the cytoplasmic or nuclear expression of β-catenin （ρ = 0.623, P 〈 0.01）, and there was a negative correlation between the cytomembrane and cytoplasmic or nuclear expression of β-catenin （ρ = -0.572, P 〈 0.01）. Conclusions There is an abnormal expression of NHERF1 and β-catenin in extramammary Paget＇s disease tissue, which may be associated with the initiation, progression, and invasion of primary extramammary Paget＇s disease.