口腔溃疡病变组织DNA分布区变化的研究

The research on the DNA distribution area change of oral ulcer lesions

目的：通过测定口腔溃疡黏膜上皮细胞，研究细胞核内DNA分布区的改变。方法：在丁卡因表麻下用小毛刷刷取1：3腔溃疡处上皮细胞，经固定后，制成1-2张玻片，行Feulgen染色做DNA倍体测定，将出现DNA分布区变化病例的溃疡组织活检行病理组织检查。结果：46例病例中有12例在口腔异倍体细胞定量测定后发现5c（7％±2．6％）、7c（11％±4．3％）和9c（16％±5％）细胞核内高DNA分布区明显较2c细胞（2％±1．2％）增多（￡一配对比较，P〈0．05）。12例病例病理证实7例中度异常增生、4例异常增生、1例浸润癌。结论：口腔溃疡病变组织中、重度不典型增生和浸润癌的病例可出现DNA倍体异常并伴有核内高DNA分布区增大。

[Abstract] Objective：To research the DNA distribution area change in the nuclei through the determination of oral ul- cer mucous membrane epithelial cells. Method：Epithelial cells in oral ulcer were took with a small brush under surface anesthesia of tetracaine ,after being fixed,smear were made centrifugal 1-2 pieces of glass, then Feulgen stained,DNA ploidy were measured, the oral ulcer lesions which had the DNA distribution area change were made a biopsy histopatholog- ic examination. Result：The research showed that 12 cases in 46 cases after oral heteroploid cell quantitative determination found 5 c （7 %±2.6 %） 7 c （11%±4.3 %） and 9 c （16 %±5 %）. High DNA distribution area in the nuclei were significantly increased compared with 2 c cells （2 %±1.2 %） （t-paired comparison,P 〈 0.05）. 12 cases were confirmed by pathology that 7 cases were moderately abnormal hyperplasia,4 cases were abnormal hyperplasia and 1 case was invasive cancer. Conclu- sion：The cases of severe atypical hyperplasia and invasive cancer in oral cavity ulcer lesions have the expression of abnor- mal DNA ploidy and high DNA area increased in nuclear.