正常唾液及氟化物对脱矿年轻恒牙牙釉质的再矿化作用

The remineralization of normal human saliva and fluoride for adolescent enamel etched by carbonated drinks

目的:用显微硬度测量法研究正常人体唾液及氟化物对碳酸饮料酸蚀的年轻恒牙牙釉质的再矿化作用。方法:收集口腔正畸门诊减数拔牙所拔除的新鲜、健康年轻恒牙23颗,制成牙釉质样本90块,随机分为对照A组、实验B组及实验C组;每组又分为三个亚组(A1、A2、A3、B1、B2、B3、C1、C2、C3),其中A1、B1、C1浸泡在雪碧中1周、A2、B2、C2浸泡在芬达中1周、A3、B3、C3浸在百事可乐中1周,每组10个牙块。A组牙浸泡完后直接用显微硬度仪做表面硬度测试;B组牙用蒸馏水冲洗干净后分别浸泡于装有正常人体唾液的容器中,将容器置于37℃恒温箱中,定期摇动容器,1月后取出用显微硬度仪做表面硬度测试;C组牙则用蒸馏水冲洗干净后,每日早中晚3次用含氟牙膏涂布牙齿开窗处表面10 min,再分别放入装有正常人体唾液的容器中,将容器置于37℃恒温箱中,定期摇动容器,1月后取出用显微硬度仪做表面硬度测试。结果:正常人体唾液及氟化物均能使脱矿后年轻恒牙牙釉质表面硬度明显升高(P<0.05),且两者之间有统计学差异(P<0.05)。结论:正常人体唾液及氟化物对脱矿年轻恒牙的再矿化作用明显。

Objective：To study the remineralization of normal human saliva and fluoride for adolescent enamel etched by carbonated drinks. Method：23 healthy adolescent teeth were collect in Orthodontics outpatient and 90 enamel samples were made. All of enamel samples were divided into 9 groups that consist of 10 enamel samples. Control group A was divid- ed into A1, A2 and A3. Experimental group was divided into B and C,Group B was divided into B1, B2 and B3,group C was divided into C1,C2 and C3. A1,B1 and C1 were soaked into Sprite in one week. A2,B2 and C2 were soaked into Fenda in one week. A3,B3 and C3 were soaked into Pepsi in one week. After that, Group A were did surface hardness testing by mi- cro-hardness tester. Group B were soaked into normal human saliva in one month and then did surface hardness testing by micro-hardness tester.Vickers hardness tester was used to determine the hardness of Group C which were coated with fluo- ride everyday and were soaked into normal human saliva in one month. SPSS17.0 was used to analyze the outcomes. Result： Normal human saliva and fluoride can make the surface hardness of demineralized adolescent enamel higher （P 〈0.05） and the difference between normal human saliva and fluoride was significant. Conclusion：The effect of remineralization of nor- mal human saliva and fluoride for demineralized adolescent enamel was significant.