大鼠非酒精性脂肪肝中UCP2动态表达研究

Study on dynamic expression of UCP2 in rat's non-alcoholic fatty liver disease

目的探讨大鼠非酒精性脂肪肝中UCP2的动态表达，进一步明确其在非酒精性脂肪肝中的发病机制。方法选取大鼠60只进行实验，按照随机分组原则划分为观察组和对照组，观察组采用高脂饮食进行脂肪肝诱导，采用免疫组织学技术和Westernblot技术对大鼠肝组织中的UCP2进行检测，对大鼠的血清甘油三脂（TG）、丙氨酸氨基转移酶（ALT）和游离脂肪酸（FFA）含量进行检测。结果大鼠在形成非酒精性脂肪肝过程中，体内UCP2阳性细胞的含量以及TG、ALT、FAA的表达和含量都会显著增加，在高脂饮食诱导8～12周期间最为明显。结论大鼠非酒精性脂肪肝的形成与发展程度与体内UCP2表达强度呈正比例关系，UCP2的酶活性增高会导致促进脂质过氧化反应，促进脂肪肝的形成。

Objective To discuss the dynamic expression of UCP2 in rat＇s non-alcoholic fatty liver disease and specify its pathogenesis in non-alcoholic fatty liver disease. Methods 60 rats were chosen for the experiment, which were randomly divided into the observation group and control group. In the observation group, rats received the high- fat diet for the induction of fatty liver and the immune histological technique and Western blot technique were adopted to measure UCP2 in rat＇s liver and contents of triglyceride （TG）, alanine transaminase （ALT） and free fatty acid （FFA）. Results During the formation of non-alcoholic fatty liver disease, the content of UCP2 positive cells and expression and contents of TG, ALT and FFA in rats were significantly increased, which were most obvious during 8th-12th week of induction of high-fat diet. Conclusion The formation and development of rat＇s non-alcoholic fatty liver disease is proportional to the expression of UCP2. The increased enzymatic activity of UCP2 will accelerate the lipid peroxidation and promote the formation of fatty liver.