外源性转化生长因子-β1复合材料对成骨细胞的影响

目的研究载有外源性TGF-β1复合材料，在体外对小鼠成骨样细胞系（MC3T3-E1）生物活性的影响。方法将MC3T3-E1接种于96孔板中，分别加入不同浓度TGF-β1溶液（0.2ng/ml，1ng/ml，5ng/ml）和FHA、FA悬浮液的混合溶液，并设置对照组，分别培养1d、3d、5d，用酶联免疫检测仪测定OD值以检测细胞活力。结果各组细胞在3d时均出现成倍增殖，除空白组外5d时均增殖减弱。浓度为1ng/ml TGF-β1比0.2ng/ml和5ng/ml具有更强的促成骨细胞增殖作用。结论 TGF-β1与FHA、FA的混合溶液可改变成骨细胞增殖周期，使之提前出现增殖高峰，且1ng/ml TGF-β1溶液对成骨细胞具有最强的促增殖作用。

Objective Research the biological activity of exogenous TGF-β1 composite materials in vitro and its effect on signal expression of osteoblastic cell（MC3T3-E1）. Methods MC3T3-E1 was seeded in 96-well plates,and added to mixed solution with different concentrations of TGF-β1 solution（0.2,1,5 ng / ml）and FHA,FA suspension, and control groups were set up. The groups were cultured 1d,3d,5d,and then OD values measured by enzyme-linked immunosorbent assay to detect cell viability. Results Cells of each group proliferated visibly in 3d,and decreased slightly in 5d except the blank,the concentration of 1ng/ml TGF-β1 had stronger promotion than 0.2ng/ml and 5ng/ml on cell proliferation. Conclusion Mixed solution with TGF-β1 and FHA、FA suspension can alter the proliferation cycle of osteoblasts,and advance the peak of proliferation. 1ng/ml TGF-β1 solution has the strongest effect on the proliferation of osteoblasts.