副猪嗜血杆菌河南分离株的鉴定及培养特性分析

Identification and Chariterization of Haemophilus parasuis in Henan Province

为有效进行副猪嗜血杆菌(HPS)病的科学防控,本研究开展了疑似副猪嗜血杆菌病临床病料病原菌的分离鉴定、培养特性研究和致病性分析等工作。结果表明,成功分离到一株HPS,培养基中添加2种无机氮源均对该菌的生长具有促进作用,在以乳糖为碳源的培养基中HPS生长较好,不同动物来源的血清对HPS生长影响无显著性差异,27.5mg/L的烟酰胺腺嘌呤二核苷酸(NAD)即可满足HPS生长需求;药物敏感性试验显示,该菌株对某些药物具有较强的耐药性;致病性试验中,豚鼠对该分离株较敏感,具有较强的致病性。

For the effective prevention and control of Haernophilus parasuis infection, a strain of HPS was chariterizated by isolation, culture characteristics and pathogenicity. The results indicated that an HPS strain was successfully isolated. Two inorganic nitrogen sources were added to the culture medium with the promotion of the growth of the bacteria, and lactose is the better carbon source for HPS growth, differ- ent sources of animal serum for HPS growth effector showed no significant difference, and 27. 5 mg/L NAD can meet HPS growth requirements. Drug sensitivity tests showed that the strain has strong resist- ance to some antimicrobials. Pathogenicity test showed that the isolated strain had a strong pathogenicity to guinea Digs.