‘焦点’观赏凤梨的组培快繁技术研究

Vitro rapid propagation of Guzmania cv. focus

以‘焦点’凤梨的吸芽和幼嫩花序为材料,研究了其组培快繁技术。结果表明,吸芽和幼嫩花序都可以被诱导分化出不定芽,以MS为基础培养基,添加6-BA 3.0 mg/L、IBA 0.1 mg/L,可以得到最好的芽诱导效果;增殖率最高的培养基是MS+6-BA1.0 mg/L+IBA 0.01 mg/L;在1/2MS基础上,添加NAA 0.5 mg/L+IBA 0.5 mg/L可以使不定芽生根最快、最多。经过组培快繁技术得到的组培苗,移栽到由90%泥炭土与10%珍珠岩混合而成的基质中,30 d后成活率可以达到98.8%以上。

Vitro rapid propagation of Guzmania cv.Focus by axillary shoot and inflorescence culture was achieved in this study. Adventitious shoots were induced well on Murashige Skoog （MS） in response to 3.0 mg/L 6-BA and 0.1 mg/L IBM Then the axillary buds were formed perfectly in the case of 1.0 mg/L 6-BA and 0.01 mg/L IBA on the MS medium; Furthermore, all the plantlets rooted best on 1/2MS with NAA0.5 mg/L plus IBA0.5 mg/L ; Finally, these new plantlets were transplanted to the greenhouse on the medium mixed with 90% peats and 10% perlites, and over 98.8% were survived after 30 d.