遗传转化的黑曲霉植酸酶基因(phyA2)对玉米利用土壤有机磷能力的影响

Effects of Aspergillus niger phyA2 Transgenic Maize on Utilization of Organic Phosphorus in Soil

以转黑曲霉植酸酶基因(phyA2)玉米T9代纯合系及与之对应世代的阴性对照为材料,通过低磷土壤培养试验研究植酸酶基因(phyA2)对玉米利用土壤有机磷能力的影响。结果显示,在施用植酸钠和不施磷的条件下,与阴性对照相比,转基因玉米根际土壤的磷酸酶活性分别提高5.17%和5.48%,根际中等活性有机磷分别降低16.2%和28.2%;植株磷积累量分别增加140%和100%,各生长指标都明显好于阴性对照。说明遗传转化的黑曲霉植酸酶基因能提高玉米利用土壤有机磷的能力,增加玉米体内磷的积累,改善玉米的生长状况。

Homozygous phyA2 transgenic maize lines at T9 generation and the corresponding generation of negative control were grown in low-phosphorus（P） soil to investigate the ability of maize plant to acquire P from organic sources.It showed that the soil phosphatase activity increased by 5.17% and 5.48%,the moderately labile organic P decreased by 16.2% and 28.2% respectively in phyA2 transgenic maize rhizosphere,and the P accumulation of transgenic plant increased significantly by 140% and 100% respectively as compared with a control plant line when supplied with phytate as P sources and without P-fertilizer.The plant growth of phyA2 transgenic maize was better improved than that of the negative control plant.These data indicated that we can improve the ability of plant to utilize soil organic P,the plant P accumulation and the plant growth by transferring Aspergillus niger phyA2 gene into maize.