摘要
4-乙烯基愈创木酚是以小麦类为原辅料的上面发酵啤酒的典型香气成分。利用该物质荧光特性,在国内首先建立啤酒中4-乙烯基愈创木酚高效液相色谱荧光检测法,并将该方法应用于3种成品啤酒和5种发酵液的检测。所建立的方法如下:C18色谱柱;荧光检测器激发波长259 nm,吸收波长341 nm;流动相为水∶甲醇∶磷酸=399∶600∶1;流速1 mL/min。该方法相对标准偏差0.7236%,回收率91.75%～93.97%,检出限0.005 mg/L。
4-vinylguaiacol is a typical flavoring substance of beer produced by wheat. Based on the fluorescence characteristics of 4-vinylguaiacol, we firstly developed the determination of 4-vinylguaiacol in beer by HPLC-FLD at home. Such method was applied for the determination of 4-vinylguaiacol in three beer products and five kinds of fermenting liquid in our study and the process was summed up as follows： C18 column used, the wavelength of the fluorescence detector was 259 nm, the absorption wavelength was 341 nm, mobile phase H2O：CHaOH：H3PO4 = 399： 600：1, and flowing rate was 1 mL/min. RSD of such method in the experiments was 0.7236 %, the recovery was between 91.75 %-93.97 %, and the detecting limit was 0.005 mg/L.
出处
《酿酒科技》
北大核心
2013年第8期93-94,97,共3页
Liquor-Making Science & Technology
基金
农业部大麦青稞产业体系项目
编号:CARS-05
关键词
4-乙烯基愈创木酚
高效液相色谱
荧光检测
4-vinyguaiacol
high performance liquid chromatography
fluorescence detector
