摘要
目的:建立高效液相色谱法测定蒜油中的大蒜素含量。方法:蒜油样品经正己烷溶液溶解,采用C18色谱柱(4.6mm×250mm,5um),以乙腈∶水∶四氢呋喃(68∶30∶2)为流动相,流速1.0mL/min,柱温:25℃,用紫外检测器在225nm波长下测定蒜油中大蒜素含量。结果:大蒜素在16.0~80.0ug/mL质量浓度范围内具有良好线性关系(r=0.9998),最低检出限为2ng/mL,在低、中、高3个水平下的加标回收率为98.4%~99.1%,相对标准偏差(RSD%)为0.29%~0.73%。结论:该方法前处理操作简便、灵敏度高、适用范围广。
Objective: To establish HPLC method for the determination of allicin in garlic oil. Meth- ods: The garlic oil sample was dissolved by n-hexane. ClS column(4.6ram x 250mm,5um) and UV detector was used to determine allicin with the conditions that the mobile phase is acetonitrile:H20:tet- rahydrofuran(68: 30: 2) , the flow rate is 1.0mL/min and the wavelength is 225nm. Results: In the range of 16.0 - 80.0ug/mL for allicin, good linear were obtained (r = 0. 999 8 ) , the lowest detection limits were 2ng/mL, the mean recoveries at three spiked levels were between 98.4% and 99.1% with relative standard deviations (RSD) of 0. 29% -0. 73%. Conclusion: This method is simple and ac- curate with widespread utility.
出处
《贵州师范大学学报(自然科学版)》
CAS
2013年第4期81-84,共4页
Journal of Guizhou Normal University:Natural Sciences