高效液相色谱法测定蒜油中大蒜素的含量

Study on determination of allicin in garlic oil by high performance liquid Chromatography

目的:建立高效液相色谱法测定蒜油中的大蒜素含量。方法:蒜油样品经正己烷溶液溶解,采用C18色谱柱(4.6mm×250mm,5um),以乙腈∶水∶四氢呋喃(68∶30∶2)为流动相,流速1.0mL/min,柱温:25℃,用紫外检测器在225nm波长下测定蒜油中大蒜素含量。结果:大蒜素在16.0～80.0ug/mL质量浓度范围内具有良好线性关系(r=0.9998),最低检出限为2ng/mL,在低、中、高3个水平下的加标回收率为98.4%～99.1%,相对标准偏差(RSD%)为0.29%～0.73%。结论:该方法前处理操作简便、灵敏度高、适用范围广。

Objective： To establish HPLC method for the determination of allicin in garlic oil. Meth- ods： The garlic oil sample was dissolved by n-hexane. ClS column（4.6ram x 250mm,5um） and UV detector was used to determine allicin with the conditions that the mobile phase is acetonitrile：H20：tet- rahydrofuran（68： 30： 2） , the flow rate is 1.0mL/min and the wavelength is 225nm. Results： In the range of 16.0 - 80.0ug/mL for allicin, good linear were obtained （r = 0. 999 8 ） , the lowest detection limits were 2ng/mL, the mean recoveries at three spiked levels were between 98.4% and 99.1% with relative standard deviations （RSD） of 0. 29% -0. 73%. Conclusion： This method is simple and ac- curate with widespread utility.