人羊水干细胞的分离培养及鉴定

Isolation,culturing and identification of the stem cells derived from human amniotic fluid

目的:分离培养人羊水干细胞(hAFSC)并鉴定。方法:使用贴壁法分离培养羊水干细胞,细胞免疫荧光法和Western blot鉴定hAFSC。结果:分离的羊水干细胞均表达特异性标记物Oct-4、c-kit、SSEA-4、CD105。结论:成功分离及培养hAFSC,可作为种子细胞用于干细胞移植治疗肝纤维化。

Objective： To isolate,culture and identify the stem cells derived form human amniotic fluid（hAFSC）.Methods： hAFSCs were isolated and cultured with adherent method,and identified by cell immune fluorescence and Western blotting.Results： Generally,the isolated amniotic stem cells expressed specific markers of Oct-4,c-kit,SSEA-4,and CD105.Conclusion： hAFSC is successfully isolated and cultured,which can be used as the seed cells for management of hepatic fibrosis through stem cell transplantation.