肌酸激酶同工酶MB质量和活力方法学比较

Outcome comparison of detecting CKMB mass and activity

目的:评价两种不同的方法检测CKMB质量和活力结果在诊断急性心肌梗死患者中的一致性。方法:留取91例临床诊断为急性心肌梗死患者的血清标本,分别采用免疫抑制酶法和化学发光法检测CKMB活力和质量,分析活力和质量两种结果在诊断急性心肌梗死患者中的差异性。结果:CKMB质量和活力分别为(8.77±10.27)ng/ml和(23.8±23.06)U/L,数据转化后,CKMB质量为(35.9±12.48)ng/ml,活力为(23.8±23.06)U/L(t=6.415,P<0.001),CKMB质量和活力均值的差异有统计学意义。以正常参考值为标准,CKMB质量阳性为53例,阴性为38例,CKMB活力阳性为29例,阴性为62例,分布有显著性差异(P<0.001),CKMB质量阳性率显著高于活力的阳性率。结论:CKMB质量的诊断价值优于活力,临床实践中,推荐使用CKMB质量为诊断参考。

Objective： To evaluate the consistency of determining creatine kinase isoenzyme（CKMB） mass and CKMB activity in diagnosis of acute myocardial infarction（AMI） by two different techniques.Methods： The serum samples were collected from 91 clinically diagnosed AMI patients,and CKMB mass and CKMB activity were assayed respectively using immunosuppressed enzyme method and chemiluminescence technique to compare the difference for the mass and activity determined.Results： The value of CKMB mass and CKMB activity was（8.77 ± 10.27） ng / ml and（23.8 ±23.06） U/L respectively.After standardization,the mean value of CKMB mass was（35.9 ± 12.48） ng / ml,which was statistically different from the mean value of CKMB activity（23.8 ± 23.06） U / L（t = 6.415,P ＆lt; 0.001）.Based on the normal reference,positive CKMB mass was found in 53 of the 91 cases and negative in 38.Positive CKMB activity was seen in 29 compared to negative in 62,and the difference was significantly different（P ＆lt; 0.01）.Positive CKMB mass was over its positive activity.Conclusion： Determination of CKMB mass appears superior to CKMB activity in diagnosis of AMI,which shall be recommendation in clinical reference.